a) Estimate the lengths of each of the DNA fragments shown in this gel A:____________ B: ____________ C: ____________ D: ____________ E: ____________ F: ____________ b) Which letter represents the DNA fragment that is the smallest? c) Which DNA fragment is approximately the same length as the lengths of fragments D & C added together?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
- a) Estimate the lengths of each of the DNA fragments shown in this gel
A:____________ B: ____________ C: ____________ D: ____________ E: ____________ F: ____________
- b) Which letter represents the DNA fragment that is the smallest?
- c) Which DNA fragment is approximately the same length as the lengths of fragments D & C added together?
Part 4. Putting It Together
1) Consider the diagram below as well as the given information.
This diagram represents a piece of circular DNA which was cut in 4 separate reactions (4 different test tubes, each with some of this DNA in it). One digest was done with AvaI, another with ClaI, a third with EcoRV, and a fourth with ScaI. The locations of the recognition sequences for each restriction enzyme are shown along with the location of that site in bp along the circle (it goes clockwise from position 1). You run an agarose gel with a molecular weight marker in the first lane, the AvaI digest in lane 2, the ClaI digest in lane 3, the EcoRV digest in lane 4, and the ScaI digest in lane 5.
- a) Use the space below and draw out the agarose gel described above. Use your drawing to answer the next questions.
b) How many bands of DNA are there in lane 3?
- c) How many bands of DNA are there in lane 5?
- d) There would be 2 bands of DNA in lane 4. How big are they?
- e) Which lane contains the largest band of DNA? How big is it?
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