A gram-negative prototrophic bacterium, Wisconsin badgerii, is growing on glutamate as a sole source for carbon, nitrogen and energy. It transports alanine via a H coupled symporter system. The bacterium has a H-FoFIATPase. [THREE PAGE LIMIT FOR THIS QUESTION] A.) Give a brief description of how alanine gets inside the cell and how the transport system operates. In your answer include how it generates the energy required for transport. (Remember the substrate is outside the cell; a gram-negative bacterium) B.) Describe what happens in alanine metabolism. How is it broken down or catabolized? How does this amino acid work into central metabolism for carbon and energy? C.) What happens to the amine group(s) on alanine? How is it incorporated into other amino acids? What is the effect on amino acid biosynthesis? (Hint: What about nitrogen regulation? Include this information

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1.
A gram-negative prototrophic bacterium, Wisconsin badgerii, is growing on glutamate as a sole source
for carbon, nitrogen and energy. It transports alanine via a H* coupled symporter system. The bacterium
has a H-FoFIATPase. [THREE PAGE LIMIT FOR THIS QUESTION]
A.) Give a brief description of how alanine gets inside the cell and how the transport system operates. In
your answer include how it generates the energy required for transport. (Remember the substrate is outside
the cell; a gram-negative bacterium)
B.) Describe what happens in alanine metabolism. How is it broken down or catabolized? How does this
amino acid work into central metabolism for carbon and energy?
C.) What happens to the amine group(s) on alanine? How is it incorporated into other amino acids? What
is the effect on amino acid biosynthesis? (Hint: What about nitrogen regulation? Include this information
in your explanation)
D.) What anapleurotic pathways are required for aerobic growth on alanine? Think about switching from
glucose to alanine
E.) If this bacterium is a facultative anaerobe, can it grow on alanine under anoxic conditions? Please
justify your answer.
Transcribed Image Text:1. A gram-negative prototrophic bacterium, Wisconsin badgerii, is growing on glutamate as a sole source for carbon, nitrogen and energy. It transports alanine via a H* coupled symporter system. The bacterium has a H-FoFIATPase. [THREE PAGE LIMIT FOR THIS QUESTION] A.) Give a brief description of how alanine gets inside the cell and how the transport system operates. In your answer include how it generates the energy required for transport. (Remember the substrate is outside the cell; a gram-negative bacterium) B.) Describe what happens in alanine metabolism. How is it broken down or catabolized? How does this amino acid work into central metabolism for carbon and energy? C.) What happens to the amine group(s) on alanine? How is it incorporated into other amino acids? What is the effect on amino acid biosynthesis? (Hint: What about nitrogen regulation? Include this information in your explanation) D.) What anapleurotic pathways are required for aerobic growth on alanine? Think about switching from glucose to alanine E.) If this bacterium is a facultative anaerobe, can it grow on alanine under anoxic conditions? Please justify your answer.
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