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A) How many origins of replication do you think the viral genome has, and why do you think this? B) Do your data support unidirectional or bidirectional replication of the viral genome? How have you come to this conclusion?

A) How many origins of replication do you think the viral genome has, and why do you think this? B) Do your data support unidirectional or bidirectional replication of the viral genome? How have you come to this conclusion?

Biology 2e
Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter14: Dna Structure And Function
Section: Chapter Questions
Problem 29CTQ: DNA replication is bidirectional and discontinuous; explain your understanding of those concepts.
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Question 2. For your senior research, you end up studying the life cycle of an animal virus whose genome consists of a single circular, double-stranded DNA molecule. Your project is to define the number and location of the origin(s) of replication and to determine whether replication proceeds in one (unidirectional) or both (bidirectional) directions away from an origin. To accomplish this, you isolate many identical strands of DNA that have already been partially replicated. You cleave each piece of DNA exactly once with a restriction enzyme. You then observe the cut pieces of DNA using an electron microscope. Below is a schematic representation of what you observe. Remember that each line represents a different piece of DNA, and not a fragment of a larger piece. Using this data, answer the following questions: A) How many origins of replication do you think the viral genome has, and why do you think this? 30: F2 #3 *** E D 80 F3 54 $ 4 F4 R LL % 5 F5 T G B) Do your data support unidirectional or bidirectional replication of the viral genome? How have you come to this conclusion? A 6 MacBook Air F6 Y H & 7 ◄◄ F7 U * 00 8 J DII F8 - ( 9 K DD F9 0 ) 0 L A F10 P
Transcribed Image Text:Question 2. For your senior research, you end up studying the life cycle of an animal virus whose genome consists of a single circular, double-stranded DNA molecule. Your project is to define the number and location of the origin(s) of replication and to determine whether replication proceeds in one (unidirectional) or both (bidirectional) directions away from an origin. To accomplish this, you isolate many identical strands of DNA that have already been partially replicated. You cleave each piece of DNA exactly once with a restriction enzyme. You then observe the cut pieces of DNA using an electron microscope. Below is a schematic representation of what you observe. Remember that each line represents a different piece of DNA, and not a fragment of a larger piece. Using this data, answer the following questions: A) How many origins of replication do you think the viral genome has, and why do you think this? 30: F2 #3 *** E D 80 F3 54 $ 4 F4 R LL % 5 F5 T G B) Do your data support unidirectional or bidirectional replication of the viral genome? How have you come to this conclusion? A 6 MacBook Air F6 Y H & 7 ◄◄ F7 U * 00 8 J DII F8 - ( 9 K DD F9 0 ) 0 L A F10 P
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