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- QUESTION 6: Based on your knowledge of DNA replication, indicate the polarity of each primer.You already know that ribosomes are abundant in red blood cells. In what other cells of the body would you find them in great abundance? Why?Question 12 What is the purpose of the inverted repeats at the ends of DNA-based transposons? A. The inverted repeats are recognized by restriction enzymes that cut out the transposon B. The inverted repeats are magnets for nucleases C. The inverted repeats are the sites of transfer on the target RNA D. The inverted repeats are recognized and cut by transposase to move the transposon from its original location
- QUESTION 23 What is the most stable nitrogenous base pairing? A. A-T B. G-C C. G-A D. C-T E. A-U5’-GATCAGCTGACTGGATCCGTCCTCAACGTCAGGATCCAGCTTCAAG-3’ 1. How many cuts do you expect this enzyme to make on the above DNA and how many fragments do you expect to see on your gel? Assume that they are all different sizes.QUESTION 24 Which of the following statements regarding the structure of DNA inside cells is NOT correct? A. In a DNA duplex, the two strands of DNA contain reverse complementary base pairs. B. The double helix is held together by phosphate bonds between the purines and pyrimidines. C. In a DNA duplex, A pairs with T and G pairs with C. D. The DNA is a double helix that contains a major groove and a minor groove. E. The double helix is held together by two sugar-phosphate backbones on the outside of the double helix with paired purine and pyrimidine bases inside joined by hydrogen bonds.
- Question:- 1. ATT GAC CAA ATC CAT TGA GAC CAA What chains occur when modified DDTP thymine is added to the DNA sequence above.QUESTION 9 These are enzymes that untwist the double helix at the replication forks of replicating DNA. Helicases Stingle-strand binding proteins Topoisomerase TelomeraseQUESTION 27 Which of the following methods can be used to compare the amounts of one specific mRNA that is expressed by two different cell lines? A. Immunohistochemistry B. Western blotting C. Polymerase chain reaction (PCR) D. Immunocytochemistry
- Question 1. Suppose that the diagram below represents the genomic organization of an enzyme involved in eye pigment production in mice. Within the gene are four exons. Biochemical analysis has revealed that the active site of the enzyme is located in the C terminus of the protein. The nucleotide length of each exon and intron is shown. The dinucleotide sequence GT represents the 5’ splice site and the dinucleotide sequence AG represents the 3’ splice site. Both the 5’ and the 3’ splice sites must be present for splicing to occur. Assume that the first and second stop codons are located immediately after the first and second 5’ splice sites, respectively; the third and fourth stop codons are located near the 3’ end of exons 3 and 4, respectively; all these stop codons are in the correct reading frame. E) Suppose you isolate a mutant mouse that has white eyes. When you examine the size of the eye pigment enzyme produced by this mouse, you see that it is 400 amino acids long. Sequence…Question 1. Although we will not be doing a gel electrophoresis, data from a gel digest of a Bacillus anthrax plasmid is provided so you can do a DNA map. The Bacillus anthrax plasmid is 4000bp (4Kb) long. Note the origin position as well as the reference molecular weight markers on the gel. Two restriction enzymes, A and B, were used to obtain two individual digests, A and B. They were combined to produce the third digest. The restriction enzyme fragment pattern for the digest of Bacillus anthrax plasmid Determining the Number of Fragments How many fragments were produced by enzyme A? How many fragments were produced by enzyme B? How many fragments were produced by the combined digest (A and B)? Fragment Size Fragment size is relative to molecular weight, and must be determined by comparing the fragment distance to the molecular weight markers. The fragment size has been provided on the gel pattern for this exercise. To make a map you must determine the relative positions of the…Question #2 To isolate DNA from cells, you must disrupt the cell Therefore, you treated your cells with a lysis buffer that contains a detergent. You also heated the cells and vortexed them during this time. What chemical property does the detergent in the lysis buffer have that helps disrupt a cell membrane? Briefly explain your answer. How does vortexing and heating your samples aid in disrupting the membrane?