An ELISA was performed on the sera of three patients to detect antibody against varicella (the chickenpox). Give the titer results shown in the answers, which patient's serum had the most anti-varicella antibody? O There is not enough information to answer the question. Patient 2, dilution 1:30 Patient 1, dilution 1:2,000 Patient 2, dilution 1:400
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- Which of the following is the correct pairing of an immunologic test and the subject to be identified? a. indirect ELISA test/an unknown microbial antigen b. direct fluorescence antibody test/an unknown antibody c. Western blot/an unknown microbial antigen or antibody d. agglutination test/an unknown soluble microbial toxinThis virtual lab was testing for lupus. How is this same tested used to test for the presence of HIV? If the results for an HIV test were the same as in this exercise, what would they indicate about the three patients?What two S. aureus antigens are being detected with the use of this test kit?
- Which of the following is TRUE about the Latex Agglutination test? Question 1 options: A Antibodies and antigens react and cannot be detected with the naked eye B Specific antigens are attached to latex particles C Is used to detect antigens in a patient's serum D Antibodies can only be detected in serum E Antigens cannot be used to detect a specific antibody Which of the following is NOT TRUE about immunological tests? Question 2 options: A Antibodies can be used to detect specific antigens B An agglutination reaction is the reaction of an antibody with a soluable antigen C An agglutination reaction is the result of a particulate antigen with a specific antibody D Based on reactions between antigen and antibody E Precipitation reactions cannot be seen with the naked eye What can you say about a positive latex…The sandwich type of ELISA detects patient antibodies to an antigen.A. FalseB. TrueThe direct ELISA test requires only one antibody only one antigen both primary and secondary antibody only secondary antibody conjugated with enzyme system
- Is rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid antibody testing? Describe how antibodies are used to detect the presence of a specific disease.Which of the following is TRUE about the Latex Agglutination test? Question 1 options: a Specific antigens are attached to latex particles b Is used to detect antigens in a patient's serumWhich of the following is TRUE about the Latex Agglutination test? Question 1 options: A Antibodies and antigens react and cannot be detected with the naked eye B Specific antigens are attached to latex particles C Is used to detect antigens in a patient's serum D Antibodies can only be detected in serum E Antigens cannot be used to detect a specific antibody
- When a patient exhibits a four-fold or greater rise in antibody titer against a specific microbial antigen between acute-phase and convalescent-phase serum samples, this is considered to be a “seroconversion”. Which of the following would be a correct interpretation of a seroconversion? what option below is the answer? None of the other four answers are a correct interpretation of a seroconversion These results show that the microbe itself has been detected in the patient’s sample(s) These findings cannot be interpreted because antibodies aren’t specific enough for antigens There is no evidence of current active infection because that would require that the antibody titer is decreasing rather than increasing The results show evidence of current or recent infection by the specific microbe because there has been an active immunologic response to its antigen7 mL of a 1:50 dilution is needed to run a specific serological test. How much serum and how much diluent are needed to make this dilution? A 1:5 dilution of patient serum is necessary to run a serological test. There is 0.2 mL of serum that can be used. What amount of diluent is necessary to make this dilution using all of the serum?What is wrong with this statement: “Prior to vaccination, the patient’s skin was sterilized with alcohol’’? What would be the more correct wording?