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- On agar plate does each discrete colony represent the growth of one cell? Explain your answer. Why can a single colony on a plate be used to start a pure culture?Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.How is the correct way of incubating culture plate media? Explain why is that so.
- During media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar plate, what is the final dilution?What is the main difference between plates before and after centrifugation? What may be the purpose of concentrating the media and perform a second plating?
- Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to growIf you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagramKligler’s iron agar and SIM are multiple test media.. What component of both media allows you to detect the preceding characteristic?
- Assume you have a stock culture at 5 x 109 cells/mL and you wish to inoculate 1 liter of fresh medium so that in 15 hours the cell density will be 2 x 108/mL. Assume a generation time of 3.5 hours. What should be the dilution?Your instructor asks you to isolate and identify the organisms in an unknown culture. You find that the culture contains two gramnegative bacilli that produce swarming colonies. What biochemical test would you use to identify the bacilli? Justify your answer.If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperation