BAPNA ΒΑΡΝΑ Abs @410 [p-nitroanil [p-nitroanil nm (mm) 0 0.2625 0.525 1.05 2.1 4.2 (mm) 0 0.0656 0.1312 0.2625 0.525 1.05 0 0.495 0.416 0.526 0.655 0.653 ine] (M) ine] (nmol) 0 0 0.00005625 62.5 0.00004727 47.27 0.00005977 59.77 0.00007443 74.43 0.0000742 74.2 [p-nitroanil ine] (nmol/min) 0 6.25 4.727 5.977 7.443 7.42 1/S (mm) (nmol/ -1 mi n) n/a n/a 15.24 0.16 7.62 3.80 1.90 1/V 0.95 0.211 0.167 0.134 0.134

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter6: Oral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 9.4P
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Can you graph the data
ΒΑΡΝΑ
(mm)
0
0.2625
0.525
1.05
2.1
4.2
ΒΑΡΝΑ Abs @410 [p-nitroanil [p-nitroanil [p-nitroanil 1/S 1/V
nm
ine]
(nmol/min)
(mm)
0
0.0656
0.1312
0.2625
0.525
1.05
0
0.495
0.416
0.526
0.655
0.653
ine]
(M)
0
0
0.00005625 62.5
ine]
(nmol)
0.00004727 47.27
0.00005977 59.77
0.00007443 74.43
0.0000742
74.2
0
6.25
4.727
5.977
7.443
7.42
(mM) (nmol/
mi n)
n/a
0.16
n/a
15.24
7.62
3.80
1.90
0.95
0.211
0.167
0.134
0.134
Transcribed Image Text:ΒΑΡΝΑ (mm) 0 0.2625 0.525 1.05 2.1 4.2 ΒΑΡΝΑ Abs @410 [p-nitroanil [p-nitroanil [p-nitroanil 1/S 1/V nm ine] (nmol/min) (mm) 0 0.0656 0.1312 0.2625 0.525 1.05 0 0.495 0.416 0.526 0.655 0.653 ine] (M) 0 0 0.00005625 62.5 ine] (nmol) 0.00004727 47.27 0.00005977 59.77 0.00007443 74.43 0.0000742 74.2 0 6.25 4.727 5.977 7.443 7.42 (mM) (nmol/ mi n) n/a 0.16 n/a 15.24 7.62 3.80 1.90 0.95 0.211 0.167 0.134 0.134
a) The Michaelis-Menten plot
Using the graph paper provided, plot the velocity of the reaction (v) against the concentration
of BAPNA substrate ([S]). Fit a curve to the data and estimate the Vmax and Km.
How would you describe the relationship between the amount of product formed and the
substrate concentration? For example, would you say that it is a linear, hyperbolic or
exponential relationship?
Transcribed Image Text:a) The Michaelis-Menten plot Using the graph paper provided, plot the velocity of the reaction (v) against the concentration of BAPNA substrate ([S]). Fit a curve to the data and estimate the Vmax and Km. How would you describe the relationship between the amount of product formed and the substrate concentration? For example, would you say that it is a linear, hyperbolic or exponential relationship?
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