5) Using FRAP (Fluorescence Recovery After Photobleaching), you can measure the diffusion rate of membrane proteins. You attach a fluorescent marker to your protein of interest, bleach a small region of the cell membrane with an intense laser light, and determine the time it takes for the bleached spot to recover fluorescent signal (see figure below). (a) (b) Bleach Laser bleaching of fluorescent marker Fluorescence intensity recovery Recovery Time How would the recovery time of your protein change if the membrane contained a higher concentration of unsaturated fatty acids? Why? How would the recovery time of your protein change if you conducted the experiment at a lower temperature? Why? How would the recovery time of your protein change if it were anchored to the membrane skeleton? Why?

Human Anatomy & Physiology (11th Edition)
11th Edition
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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5) Using FRAP (Fluorescence Recovery After Photobleaching), you can measure the diffusion
rate of membrane proteins. You attach a fluorescent marker to your protein of interest, bleach a
small region of the cell membrane with an intense laser light, and determine the time it takes for
the bleached spot to recover fluorescent signal (see figure below).
(a)
(b)
Bleach
Laser bleaching
of fluorescent
marker
Fluorescence
intensity
recovery
Recovery
Time
How would the recovery time of your protein change if the membrane contained a higher
concentration of unsaturated fatty acids? Why?
How would the recovery time of your protein change if you conducted the experiment at a lower
temperature? Why?
How would the recovery time of your protein change if it were anchored to the membrane
skeleton? Why?
Transcribed Image Text:5) Using FRAP (Fluorescence Recovery After Photobleaching), you can measure the diffusion rate of membrane proteins. You attach a fluorescent marker to your protein of interest, bleach a small region of the cell membrane with an intense laser light, and determine the time it takes for the bleached spot to recover fluorescent signal (see figure below). (a) (b) Bleach Laser bleaching of fluorescent marker Fluorescence intensity recovery Recovery Time How would the recovery time of your protein change if the membrane contained a higher concentration of unsaturated fatty acids? Why? How would the recovery time of your protein change if you conducted the experiment at a lower temperature? Why? How would the recovery time of your protein change if it were anchored to the membrane skeleton? Why?
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