From a soup sample, 1 mL of sample is taken and diluted in 99 mL of soup medium. culture, later, this suspension is analyzed in the Petroff-Hausser chamber, It is determined that the total number of bacteria found is 53 bacteria per square, if the chamber has 25 squares, how many bacteria are found in the chamber? initial juice sample?
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From a soup sample, 1 mL of sample is taken and diluted in 99 mL of soup medium. culture, later, this suspension is analyzed in the Petroff-Hausser chamber, It is determined that the total number of bacteria found is 53 bacteria per square, if the chamber has 25 squares, how many bacteria are found in the chamber? initial juice sample?
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- 1 mL of a juice sample is taken and diluted in 9 mL culture medium, subsequently, this suspension is analyzed in the Petroff-Hausser chamber, determines that the total number of bacteria found is 44 bacteria per square, if the chamber has 25 squares, how many bacteria are in the chamber? initial juice sample?You were asked to prepare a dilution series of a bacterial culture where only 3 tubes will be used (all with 5 mL total solution). Draw a schematic diagram to make tube 1 have a 10-2 dilution, tube 2 have 10-3 dilution, and tube 3 to have 10-5 dilution You were instructed to dilute an antibiotic solution 1/10, redilute 1/25, and again 1/50, then you need to make 100mL of each dilution. How would you go about preparing this dilution series? (Present your answer in a schematic diagram)You have a culture of bacteria with an unknown number of bacteria in it. You have at your disposal 10 1-mL pipettes, 3 dilution bottles that hold up to 100mL of volume, 3 test tubes that hold up to 10mL of volume, a graduated cylinder, an unlimited supply of distilled water. Explain how you would set up a 1: 10,000,000 dilution of your culture.
- One ml of a water sample was placed in a Petri dish with melted agar. After incubation, 129 colonies were counted. How many bacteria (CFU/ml) are in the sample? Thank youA 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 278 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture? Express your answer to two decimal places using scientific notation. In scientific notation 540 would be written as 5.40*10^2. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transferred, a dilution is being performed. Any time more than 1 mL is transferred, a concentration is being performed. Include the trailing zero so there are two decimal places Canvas expects a single digit before the decimal point. 5.40*10^2 is how Canvas expects 540 to be formatted in scientific notation 54.00*10^1 would be marked wrong. 0.54*10^3 would be marked wrong. A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the…In this activity, you will receive an “unknown” containing two species. The first step in identifying these bacteria is to __________. In this activity, you will receive an “unknown” containing two species. The first step in identifying these bacteria is to __________. inoculate a glucose fermentation tube perform an acid-fast stain streak a TSA plate for isolation inoculate two TSA slants inoculate two OF-glucose tubes
- When you interpret a Gram-stained smear, you should also describe the morphology (shape) of the cells, and their arrangement. In the figure below, there are two distinct types of bacteria, distinguishable by Gram stain reaction, and also by their shape and arrangement. Below, describe these characteristics for both bacteria: Gram positive bacterium Gram negative bacterium Morphology cocci bacillus ArrangementSuppose you are to observe an unknown bacterial slide. In your initial observation, it appears to be in fading color and the shape of the specimen seems to be diffused. How are you going to troubleshoot the focusing of the specimen for you to identify clearly the bacteria?You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix. You take 1 ml from bottle A and transfer it to bottle B, which contains 99 ml of sterile water. What is the dilution of the bacterial culture in bottle B?
- Your broth culture contains 471,612,048 bacteria/mL. Design a dilution series that would result in a plate with a countable number of colonies.You prepared 10-7, 10-8, and 10-9 dilutions of a bacterial suspension in sterile saline. Then you plated 0.15mL if each dilution onto each of 3 plates of nutrient agar. After incubation, the colonies were way too numerous to counton the plates prepared from the 10-7 dilution. The plates from the 10-8 dilution had 355, 360, and 350 colonies. The platesfrom the 10-9 dilution had 115, 117, and 118 colonies. Determine the concentration of colony forming units (cfu/mL) inthe original bacterial suspensionThe students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?