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Briefly explain why this is ELISA referred to as colorometric assay.
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Solved in 2 steps
- Explain why a semi-log plot should be used for determining antigen concentration by ELISA.The elimination of several steps in the ELISA could be accomplished if the primary antibody was made into an enzyme conjugate. Why is this generally not done? What can cause a false positive in an ELISA?What are ELISA assays used for in labs? Give at least three examples.
- Describe the mechanism of an Indirect ELISA. Why is ELISA so sensitive? Why is it necessary to block unoccupied binding sites in the microtiter wells? Why is it important to have a positive control?ELISA requires the use of blocking solution; what is the purpose of the solution?Conjugated enzymes are a key component of ELISA. To what are these enzymes conjugated, and why is that important? To the antigen so the enzymes are only present when the molecule of interest is also present To the plastic of the reaction well so the enzymes can concentrate the molecule of interest To the capture antibody so the enzymes are only present when the molecule of interest is also present To the detection antibody so the enzymes are only present when the molecule of interest is also present