Can you please explain what is happening in this figure? Please address controls, experimental method, what conclusions can be drawn and why the data supports these conclusions, and anything else... This data is taken from this article https://www.nature.com/articles/nature13994Thank you so much aSTARR-seq constructORFhkCPordCPEnhancerORFhkCP S2STARR-seq1,8224,134 (32%) 3,586pA siteTCTTATA box MTE DPEInrdCP S2STARR-seqAAAAAddCP S2 STARR-seqenrichment (log₂)6b-2CG33936Fold change<2x hkCP and dCP2x-4x hkCP.2x-4x dCP.>4x hkCP •>4x dCP04hkCP S2 STARR-seqenrichment (log₂)CG3393726Enr. rep2 (log₂)CG18473CG8129Enr. rep2 (log₂)Fps85D-hkCP S2 STARR-seqhkCP S2 STARR-seqdCP S2 STARR-seqEnr. rep1 (log₂)dCP S2 STARR-seqEnr. rep1 (log₂)ePer cent positiveper tested40200Luciferase-pA sitehkCP luciferase1008060Luciferase construct121/241/24hkCPenhancerOr85f2/12dCPCG34117CG8132enhancerCG81354CG9356RpL34bEnhancercandidatedCP luciferase10/12 6/7 6/7|||hkCP DCPorare SiFigure 1 Distinct sets of enhancers activatetranscription from the hkCP and dCP in S2 cells.a, STARR-seq set-up using the hkCP housekeeping(RpS12; purple) and dCP developmental corepromoters (Drosophila synthetic core promoter(DSCP)¹¹; brown) b, Genome browser screenshotdepicting STARR-seq tracks for both corepromoters. c, Overlap of hkCP and dCP enhancers.d, hkCP versus dCP STARR-seq enrichmentsat enhancers (insets show enrichment for replicates(Enr. rep) 1 versus 2 for hkCP and dCP; dCPdata reanalysed from ref. 12). e, hkCP, dCP orshared enhancers that activate luciferase(>1.5-fold and P<0.05 (one-sided t-test); n = 3;Extended Data Figs 3 and 5) from hkCP (purple)or dCP (brown; numbers show positive/tested).

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Can you please explain what is happening in this figure? Please address controls, experimental method, what conclusions can be drawn and why the data supports these conclusions, and anything else... This data is taken from this article https://www.nature.com/articles/nature13994

Thank you so much

a
STARR-seq construct
ORF
hkCP
or
dCP
Enhancer
ORF
hkCP S2
STARR-seq
1,822
4,134 (32%) 3,586
pA site
TCT
TATA box MTE DPE
Inr
dCP S2
STARR-seq
AAAAA
d
dCP S2 STARR-seq
enrichment (log₂)
6
b
-2
CG33936
Fold change
<2x hkCP and dCP
2x-4x hkCP.2x-4x dCP
.>4x hkCP •>4x dCP
0
4
hkCP S2 STARR-seq
enrichment (log₂)
CG33937
2
6
Enr. rep2 (log₂)
CG18473
CG8129
Enr. rep2 (log₂)
Fps85D-
hkCP S2 STARR-seq
hkCP S2 STARR-seq
dCP S2 STARR-seq
Enr. rep1 (log₂)
dCP S2 STARR-seq
Enr. rep1 (log₂)
e
Per cent positive
per tested
40
20
0
Luciferase-pA site
hkCP luciferase
100
80
60
Luciferase construct
121/24
1/24
hkCP
enhancer
Or85f
2/12
dCP
CG34117
CG8132
enhancer
CG81354
CG9356
RpL34b
Enhancer
candidate
dCP luciferase
10/12 6/7 6/7
|||
hkCP DCP
or
are Si
Figure 1 Distinct sets of enhancers activate
transcription from the hkCP and dCP in S2 cells.
a, STARR-seq set-up using the hkCP housekeeping
(RpS12; purple) and dCP developmental core
promoters (Drosophila synthetic core promoter
(DSCP)¹¹; brown) b, Genome browser screenshot
depicting STARR-seq tracks for both core
promoters. c, Overlap of hkCP and dCP enhancers.
d, hkCP versus dCP STARR-seq enrichments
at enhancers (insets show enrichment for replicates
(Enr. rep) 1 versus 2 for hkCP and dCP; dCP
data reanalysed from ref. 12). e, hkCP, dCP or
shared enhancers that activate luciferase
(>1.5-fold and P<0.05 (one-sided t-test); n = 3;
Extended Data Figs 3 and 5) from hkCP (purple)
or dCP (brown; numbers show positive/tested).

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