Consider a mutant of E. coli that has an inactivating mutation in the gene for adenylate cyclase (cya). Choose the compound(s) that could be provided to the mutant to achieve full transcription of the lac operon. If no combination of compounds can allow for transcription, chose none. O ATP O CAMP Oglucose O lactose | none

Consider a mutant of E. coli that has an inactivating mutation in the gene for adenylate cyclase (cya). Choose the compound(s) that could be provided to the mutant to achieve full transcription of the lac operon. If no combination of compounds can allow for transcription, chose none. O ATP O CAMP Oglucose O lactose | none

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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The Lac operon is a common example of prokaryotic control of gene expression. In what condition is it turned on?
An E. coli cell acquires a mutation that ablates the ability of the lac repressor to bind the inducer/operator region of the DNA. Under which condition(s) will the genes of the lac operon be transcribed at high levels? Select all that apply. Glucose absent, lactose absent Glucose absent, lactose present Glucose present, lactose absent Glucose present, lactose present
In the lac operon (below), how will expression of the genes lacZ and lacy be effect by a mutation in the repressor protein (encoded by lach) that prevents it from binding the operator sequence (lacO) in the absence of lactose? Explain the answer
. In an effort to determine the location of an operator sitefor a negatively regulated gene, you have made a seriesof deletions within the regulatory region. The extent ofeach deletion is shown by the line underneath the sequence, and the resulting expression from the operon(i = inducible; c = constitutive; − = no expression) isalso indicated.... GGAT C T T AGCCGGCTAACATGATAAATATAA......C C T AGAATCGGCCGA TTGTA C T A TTT ATAT T ...1 i2 –3 c4 –5 ca. What can you conclude from these data about thelocation of the operator site?b. Why do you think deletions 2 and 4 show no expression of the gene?
There is a mutation in sequence of the lac operator that prevents the lac repressor from being able to recognize and bind to it. What would the status of transcription of the lac operon be under each of the following circumstances? Explain your answers. a-Lactose present, glucose present b-Lactose present, glucose absent c-Lactose absent, glucose present d-Lactose absent, glucose absent
If CAP is bound to the Promoter of the Lac Operon and the repressor protein is not bound to the operator, which of the following is true: a.Glucose Levels are low & Lactose is absent b.Glucose is absent & Lactose is present c.cAMP is low & Lactose is Absent d.Glucose and Lactose are Present e.cAMP is low & Lactose is present
If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
Explain why large amounts of transcript result when a bacterium with a lac Operon is in a medium with no glucose and an abundance of lactose.
"The highest level of transcription of the E. coli lac operon occurs when CAP (+cAMP) is bound to the CAP site and LacI is not bound to the operator." The preceding statement is... True or False?
What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)
A mutant strain has a defective lac operator site that results in theconstitutive expression of the lac operon. Outline an experimentyou would carry out to demonstrate that the operator site mustbe physically adjacent to the genes that it influences. Based onyour knowledge of the lac operon, describe the results you wouldexpect.
In the lac operon, how would gene expression be affected if each one of the following segments was missing? A. lac operon promoter B. Operator site C. lacA gene