ate) DEFINE what is an Operon, and compare and contrast regulation of the LAC Operon and the TRP Operon.When is each turned ON or OFF?A. DRAW each operon in the PRESENCE of its own ligands (signal molecules).B. What controls the activity of the regulatory proteins involved?· Be sure to describe and show both POSITIVE and NEGATIVE regulation where appropriate.C. Explain HOW each type of control system is appropriate for an operon encoding either catabolic or anabolic enzymes.

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Description: ate) DEFINE what is an Operon, and compare and contrast regulation of the LAC Operon and the TRP Operon.When is each turned ON or OFF?A. DRAW each operon in the PRESENCE of its own ligands (signal molecules).B. What controls the activity of the regu

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Biology

DEFINE what is an Operon, and compare and contrast regulation of the LAC Operon and the TRP Operon. When is each turned ON or OFF?

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 13TYU: INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following...

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. a. How many ribosomes are required (at a minimum)for the translation of trpE and trpC from a singletranscript of the trp operon?b. How would you expect deletion of the two tryptophan codons in the RNA leader to affect theexpression of the trpE and trpC genes?
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If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
Consider the following scenario: A bacteria cell is surrounded by lactose, but there is no glucose readily available. Will the lac operon be active (on) or inactive (off)? Explain in your own words. If it is active, describe the levels (low or high) of transcription, and any positive regulators assisting with this. If it is inactive, describe the negative regulators assisting with this, and how the operon could be turned on. Question 48 options:
In the regulation of the tryophan operon in bacteria, the co-occurence of transcription and translation plays a key role regulating full activation of operon expression. Diagram and explain how during the conditions of high tryptophan concentration this system regulates the expression of genes in the operon.
You have isolated two different mutants (reg1 andreg2) causing constitutive expression of the emu operon (emu1 emu2). One mutant contains a defect in aDNA-binding site, and the other has a loss-of-functiondefect in the gene encoding a protein that binds tothe site.a. Is the DNA-binding protein a positive or negativeregulator of gene expression?b. To determine which mutant has a defect in the siteand which one has a mutation in the binding protein, you decide to do an analysis using F′ plasmids. Assuming you can assay levels of the Emu1and Emu2 proteins, what results do you predict forthe two strains (i and ii; see descriptions below) ifreg2 encodes the regulatory protein and reg1 is theregulatory site?i. F′ (reg1−reg2+emu1−emu2+)/reg1+reg2+emu1+emu2−ii. F′ (reg1+reg2−emu1−emu2+)/reg1+reg2+emu1+emu2−c. What results do you predict for the two strains(i and ii) if reg1 encodes the regulatory proteinand reg2 is the regulatory site?
1. If the above gene is one of the three structural genes of the lac operon that codes for theprotein/ enzyme responsible for breaking lactose into two molecules of simple sugars,a.) what triggers the activation of this gene?b.) what triggers the inactivation of this gene?c.) what substance is attached to the operator region of the operon in the absence ofactivator?d.) what gene is responsible for the synthesis of the substance used to attach in theoperator region in the absence of activator? e.) what substance is attached to the promoter region of the operon in this case?
You have isolated two different mutants (reg1 and reg2) causing constitutive expression of the emu operon (emu1 emu2). One mutant contains a defect in a DNA-binding site, and the other has a loss-of-function defect in the gene encoding a protein that binds to the site. Is the DNA-binding protein a positive or negative regulator of gene expression? Explain. To determine which mutant has a defect in the site and which one has a mutation in the binding protein, you decide to do an analysis using F′ plasmids. Assuming you can assay levels of the Emu1 and Emu2 proteins, what results do you predict for the two strains (i and ii; see descriptions below) if reg2 encodes the regulatory protein and reg1 is the regulatory site? Explain. F′ (reg1− reg2+ emu1− emu2+)/reg1+ reg2+ emu1+ emu2− F′ (reg1+ reg2− emu1− emu2+)/reg1+ reg2+ emu1+ emu2−
What is the difference between negative and positive regulation in operon? Explain it with the help of lac operon.
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