Determine the microbiology tests used to identify the microorganism(cutaneous anthrax) i. Gram stain Culture results Colony characteristics on media Diagnostic test results that identified the microorganism
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Determine the
i.
- Culture results
- Colony characteristics on media
- Diagnostic test results that identified the microorganism
Cutaneous anthrax is the most common form of anthrax infection, and it is also considered to be the least dangerous kind of infections. Cutaneous anthrax is most common on the head, neck, forearms, and hands. It affects the skin and tissue around the site of infection.
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- For Staphylococcus aureus, indicate which tests need to be done & what media you need. Fill in box provided Test Media NeededThe catalase test is used to primarily differentiate Staphylococi from _____________. A. Gram-negatives B. Microaerophiles C. Streptococci D. Anaerobesa specimen from an infected wound has been submitted to the clinical laboratory. explain in detail the steps necessary to identify the etiologic agent causing the infection .
- Oven sterilization of aqueous culture medium. Effect: Rationale:An outbreak of Methicillin-resistant Staphylococcus aureus (MRSA) in the surgical ward at the hospital forces the hospital administrator to cancel and postpone all non-essential surgeries until the source of infection has been located and for measure put into place. Bacterial samples obtained from swab of wad staff equipment and surfaces arr brought to the lab for analysis. 1. What testing method will you employ to determine the level of exposure? 2 How would a positive test present? 3. How would a negative test present? 4. What recommendations would you make in your report?Match the best selection media with the organism A. Carr B. MRS, Carr and WLD Positive C. MRS or WLD D. Lysine media a. Pediococcus damnosus b. Oenococcus oeni c. Candida vini d. Dekkera intermedium e. Lactobacillus brevis f. Acetobacter pasteurianus
- Identify the method of sterilization/ chemical agent described At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20 minutes to sterilize the material. The material to be sterilized is exposed to live steam for 30 minutes for 3 consecutive days.Identify what microorganisms are in each of the 10 plates from studying the gram stain and agar plates，and if they have any known antimicrobial resistanceA patient exhibits a boil on his neck. You, as a microbiology technician, are asked to identify the causative organism and determinewhether it is pathogenic. Describe the procedure that you would follow tomake this determination.
- Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial morphology for NA, color of colonies for EMB, and media color for MSA. Organism/ Media Nutrient Agar (NA) Eosin Methylene Blue (EMB) Agar Mannitol Salt Agar (MSA) Escherichia coli Pseudomonas aeruginosa Staphylococcus epidermidis Staphylococcus aureus Unknown 9 mixtureGive the procedure of Sabin-Feldman dye test. Describe the positive and negative results.