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- Calculate the concentration of protein in the diluted supernatant and the supernatant before dilution at different pH pH 3.5 4.5 5.5 6.5 7.5 8.5 Dilution Factor 5 5 5 50 50 50 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054Using Bradford Assay, plot the standard curve and find the unknowns. Protein Concentration A595 of sample 0 0 3 0.220 6.5 0.383 12 0.667 18.8 0.930 24.2 1.090 Unknown 1 1.085 Unknown 2 0.708 Unknown 3 0.193Can you please give me a little more detail on finding Ki? Where did the values come from? 0.23, 0.15, etc
- Enzyme chymotrypsin (enzyme digest) Samples: - 1 & 5- Standard Protein (Ladder) - 2 & 6- UNCUT (tube #1) - 3 & 7- 5 mins (tube #2) - 4 & 8- 30 mins (tube #3) Human and Bovine (Left) Chicken and Pigeon (Right) In this experiment we will be comparing the structural similarity of serum albumin from several different vertebrate species and thus examining how closely related the proteins from these species are to each other. These proteins have similar molecular weights. We will first cut the proteins with the enzyme chymotrypsin (enzyme digest). We will then separate the fragments based on their relative sizes through gel electrophoresis so that we can compare the fragments generated by the enzyme digest for the different species. Write the hypothesis and prediction for the experiment.If a pediatric vitamin contains 1500 units of vitamin A per milliliter of solution, how many units of vitamin A would be administered to a child given 2 drops of a solution from a drpper calibrated to deliver 20 drops per milliar of solution?The concentration of hydrolyzed nitrocefin at each time point for an experiment is given below. Time (min) Concentration (μM) 0.5 6.01 1 11.78 1.5 17.6 2 23.51 2.5 29.58 3 35.31 3.5 39.73 4 44.5 4.5 48.18 5 50.05 5.5 52.72 6 54.01 6.5 55.06 7 55.65 7.5 56.39 8 56.74 8.5 57.49 9 58.03 9.5 58.61 10 58.69 Make a graph that plots the concentration of hydrolyzed nitrocefin (in μM) against time (in seconds) using Excel, R, SPSS or other computable software. Where appropriate, include a trendline that shows the linear range on your graph. Include the equations for the trendlines and the R2 value on the graph. Your graph should also include a title and appropriate titles for the x- and y-axes, with units included where appropriate. To determine the initial velocity of a possible insert in this experiment, you must determine what the linear range is in these data (see note below). Based on your graph, which time points represent a suitable linear…
- The non linear part of the Standard curve graph can be used for estimating the unknown sample protein concentration? True FalseThe adult RDA of riboflavin is 1.3 mg. If one glass (100 mL) of apple juice contains 0.014 mg of riboflavin, how much apple juice would an adult have to consume to obtain the RDA?After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.
