Explain the limitations of the following expressions: (a) DS = C ln(Tf /Ti), (b) DG = DH − TDS, and (c) DG = wmax,non-exp.
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- Using the Nernst equation, calculate the equilibrium potential for Ca2 and for C1 from the following sets of data: a. Given [ Ca2+ ]0=1mM,[ Ca2+ ]i=100nM, find Eca2+ b. Given [ Cl- ]0=110mM,[ Cl- ]i=100mM, find EclWhy should the ethanol/lysate boundary not be disturbed?What can Kd values be used for?
- why is there a difference between the stock solution concetration of chymotrypsin calculated from zero time y intercepts versus the stock solution label information?Discuss the relationship of Lineweaver-Burke and Michaelis Menten equations. What are the importance of the double reciprocal plot, Michaelis constant, and Vmax?At what concentration of S(expressed as a multiple of KM) will νo=0.95Vmax?
- The graph of the Michaelis-menten equation, vo against [So] is unsatisfactory as a means of determining Vmax and Km. Briefly discuss why this is the case. How did lineweaver and buck overcome this problem?What is the michaeli's equation??In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the absorbance of the serum sample is determined as 0.28, what is the amount of cholesterol in the sample, in mg/L?
