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Explain the reason why the treatment of trypsin, collagenase, and EDTA is necessary for the isolation of cell.
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- Explain why culture based biochemical and genetic testing would not be the ideal method to identify the microbe.Explain how an enzyme can be useful in treating a bacterialinfection, such as that which occurs with cystic fibrosis.Explain why sulfa drugs prevent bacterial growth without harming the human host.
- Why is it necessary to inoculate the KIA medium all the way down to the bottom of the tube during inoculation?why is Lysozyme treatment useful in bacterial cell lysis when protien isolation is happening.E. coli O157:H7 is an organism of concern in contaminated foods. How can the use of MacConkey agar and Sorbitol-MacConkey agar be used to screen for E. coli bacteria in general and E. coli O157:H7 in particular?
- Why is it important for a microbiology laboratory technician to use aseptic technique?Give advantages and disadvantages of using commercially available kits for plasmid extraction over conventional alkaline lysis technique.You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures ,State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminated