Explain, using the pCR 2.1 vector, what white colonies and blue colonies mean?

Q: Describe how it is that when a short tandem repeat (STR) genomic region is PCR-amplified you can get…

A: * PCR is known as Polymerase chain reaction , a method which is used to make billions of copies of…

Q: Give at least four(4) uses/application of PCR. Explain.

A: PCR (Polymerase Chain Reaction) is use to amplify DNA. It can make billions of copies of a target…

Q: If the RT-PCR test is negative, give instances when repeat RT-PCR test should be done. If repeated,…

A:

Q: After 30 PCR cycles, there will be more than 1 billion short products derived from each starting…

A: Polymerase chain reaction (PCR) is the procedure by which a specific region of DNA is amplified to…

Q: a. Explain whether or not DNA polymerase from a mesophilic bacterium could be used successfully in a…

A: A)- In a mesophile optimum condition would allow the microbe to replicate at it maximum amount…

Q: Can the Ames test detect all mutagenic chemicals? What application does ithave in the pharmaceutical…

A: Biology is the scientific approach to study life. All the living forms share certain key features…

Q: diiferentiate  between PCR and Sanger sequencing?

A: PCR (polymerase chain reaction is a technique through which a DNA segment is copied to million folds…

Q: How does PFGE separate larger fragments more efficiently than standard electrophoresis? 2. Why is…

A: Note-We are supposed to answer only 1 question as per Bartleby guidelines. Kindly, repost the other…

Q: what are the variants of PCR ?

A: PCR ( polymerase chain reaction) is a test tube method for amplifying a selected DNA sequence. There…

Q: 1- Describe 2 approaches that you can use to determine that you have successfully PCR amplified…

A: PCR or polymerase chain reaction is used to detect genetic material (DNA) from a specific organism.…

Q: Why is it necessary to chelate the metal ions from the solution during the boiling/lysis step at 100…

A: The polymerase chain reaction (PCR) is a fundamental biochemical method for exponentially…

Q: The GoTaq Green Master Mix  contains Taq polymerase, dNTPs, and MgCl2.  What is the function of each…

A: PCR or polymerase chain reaction is a process used in biotechnology and molecules in which small…

Q: If possible please answer all questions based on the gel electrophoresis: 1. What is PCR product…

A: Answer: 1] PCR product size:                               PCR ~ Polymerase Chain Reaction…

Q: Explain about quantitative real-time PCR (qPCR) ?

A: PCR is a method used to form millions to billions of copies of a particular sample of DNA. It allows…

Q: Draw a diagram of what occurs in PCR during the annealing step of the FIRST cycle. b. Draw a diagram…

A: The PCR or polymerase chain reaction is a routinely used technique in molecular biology. It is used…

Q: In the second step of the PCR, the temperature is reduced to between 45-65 degrees C. What happens…

A: Polymerase chain reaction (PCR) is considered a molecular technique to produce multiple copies of a…

Q: Describe 2 approaches that you can use to determine that you have successfully PCR amplified Sonic…

A: A gene is a genetic molecule that is passed down from one generation (parents) to the next…

Q: What IS THE difference between Sanger sequencing and Illumina sequencing?

A: Sanger sequencing, also known as the “chain termination method”, is a method for determining the…

Q: What is the first step that should be done in PCR testing?

A: PCR is the polymerase chain reaction. PCR test or polymerase chain reaction test is good method to…

Q: Why Touch-down PCR technique is better than the Gradient PCR technique? Also, from those techniques…

A: The PCR or polymerase chain reaction is a technique to enhance or amplify DNA sequences in vitro by…

Q: Explain about Multiple PCR cycles ?

A: PCR stands for a polymerase chain reaction in which multiple copies of DNA molecules can be obtained…

Q: Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the…

A: Introduction Sickle cell disorder is a rare condition in which the red blood cells of the individual…

Q: It is possible to diagnose genetic mutation by PCR. Explain

A: The process of finding unknown or known mutations in the genes or genetic material is known as…

Q: How does diagnostic method for detecting Covid-19 using RT-PCR work?

A: RT–PCR also called Real-time PCR (polymerase chain reaction) is a nuclear-derived method or test…

Q: What is the addition and limitation of the RT-PCR approach? Keep CoViD-19 in mind

A: Viruses are a nucleoprotein entity which is able to utilize the synthetic machinery of a living cell…

Q: What 4 components are needed for PCR to occur?

A: Polymerase chain reaction used to make thousands of copies of DNA from very small amount of the DNA…

Q: When using a micro centrifuge, what should you check before starting the machine? A. That the…

A: Microcentrifuge is a type of laboratory centrifuge, which is used to separate particles in…

Q: How does RT-PCR differ from traditional PCR?

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: Explain the importance of thermostable polymerase used for PCR ?

A: Introduction: Biotechnology is a discipline of science concerned with the use of biological methods…

Q: The best combination of regeants for a typical PCR reacion would include the following concentration…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: What are sonme applications of PCR?

A: According to guidelines we have to answer the first question only. so please kindly post the…

Q: How does the shotgun approach differ from the whole genome sequencing?

A: Biotechnology is concerned with exploitation of biological agents or their components for generating…

Q: Arrange the following steps in the PCR experiment 1 3 5. Add samples and reagents Extend Anneal…

A: (According to our guidelines we are required to answer the first question in case of multiple…

Q: How many DNA molecules would exist after 5 PCR cycles?

A: PCR or polymerase chain reaction is an in vitro DNA amplifying technique. It is a very popular…

Q: In the third step of the PCR, the temperature is raised to 73 degrees C.  What happens during this…

A: PCR: PCR (polymerase chain reaction) is a technique used to make multiple copies of a particular…

Q: In sequential order, what are the three steps of PCR?   A. Denature DNA, Anneal Primers, Extend…

A: Polymerase (PCR) chain reaction, is a process during which thousands of copies of the DNA can be…

Q: Vhat is needed from the cells for PCR?

A: The polymerase chain reaction (PCR) is a technique for replicating a piece of DNA millions of times.…

Q: QUESTION 4: Should PCR primers be complementary to each other?

A: Answer 4-:PCR primers should be complementary to the strands of DNA rather than complementary to…

Q: w to achieve carbon dioxide requirement for capnophilic organisms? What is the used of “Thermus…

A: Capnophiles are microbes that can survive in the presence of higher carbon dioxide concentration.…

Q: Explain the Detection of the sickle-cell mutation by sequencing of PCR products?

A: The disorder in which a group of red blood cells becomes sickle-shaped, it is known as sickle cell…

Q: Describe the three steps of a PCR cycle.

A: Polymerase chain reaction is a molecular biology technique used to amplify a single copy of DNA that…

Q: at is the purpose of the PCR technique in Covid testing?

A: The question is all about the PCR technique i.e polymerase chain reaction technique. It is the…