Has 53' DNA polymerase activity [Choose] [Choose ] RNA polymerase DNA polymerase I Coats single-stranded DNA, prohibiting from beta subunit (sliding clamp) pairing with itself SSB (ssDNA-binding protein) DnaA protein Rho factor topoisomerase Has 5' 3' DNA exonuclease activity Seals nicks in DNA, using ATP Relaxes supercoiled DNA in front of replication fork Unwinds DNA double helix, using ATP hydrolysis Synthesizes RNA primer Binds to sequences in the origin of replication, unwinding the DNA, and allowing the helicase to be loaded Increases processivity of DNA pol III DnaB helicase Okazaki fragment Terminator primase DNA ligase [Choose] [Choose]
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
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![For each of the properties/functions below, put the protein that this describes from the list.
Each match
Has 5' 3' DNA polymerase activity
[Choose ]
[Choose]
RNA polymerase
DNA polymerase I
Coats single-stranded DNA, prohibiting from beta subunit (sliding clamp)
pairing with itself
SSB (ssDNA-binding protein)
DnaA protein
Rho factor
topoisomerase
Has 5' 3' DNA exonuclease activity
Seals nicks in DNA, using ATP
Relaxes supercoiled DNA in front of
replication fork
Unwinds DNA double helix, using ATP
hydrolysis
Synthesizes RNA primer
Binds to sequences in the origin of
replication, unwinding the DNA, and allowing
the helicase to be loaded
Increases processivity of DNA pol III
DnaB helicase
Okazaki fragment
Terminator
primase
DNA ligase
[Choose ]
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