How does the differences in the physical and chemical structure of Gram-positive and Gram-negative bacteria help in the laboratory identification of groups of microbes?

Can you classify household disinfectants as sterilants? What organisms (if any) survive after the use of common household disinfectants that would not survive the use of sterilization/aseptic technique? List a minimum of two organisms that would survive.

Because elemental poisons, such as heavy metals, cannot be furtherdegraded even by microbes, what is a possible fate of these metals?

Describe what three strategies are used to identify microorganisms?

Ex. 43  = Evaluating the Effectiveness of Common Antiseptics and Disinfectants (p. 397) What is the difference between an antiseptic and a disinfectant? What is the difference between a bactericidal and a bacteriostatic compound? How can you determine which species are sensitive to an antiseptic?   Ex. 44  = Antibiotic Evaluation by the Kirby-Bauer Method (p. 405) Why is it important to standardize this test? What parts of the test are standardized? What makes the Mueller Hinton agar especially useful for this test? How can you determine if an organism is sensitive/resistant to an antibiotic? Understand the relationships between Kirby Bauer and antibiotic susceptibility/resistance.

List three differences between gram positive and gram negative bacteria besides the color difference when they are stained.

What conditions can cause gram-positive bacteria to appear as gram-negative?

Can an object be considered clean with bacteria on it?

Why is it so important to develop tests to ID bacteria

How might the physical differences between gram-positive and gram-negative bacteria contribute to differences in chemical resistances?