How might a frame shift mutation in the lacy region influence the phenotype of this bacteria when glucose is abundant in the cell? • How would a missense mutation in lacl alter the amount of B-Galactosidase present in the cell? ● If wild type E. coli were exposed to a growth medium containing glucose, lactose and cyclic adenosine monophosphate, what would happen to the lac operon? Repressor gene lacl Regulatory region Promoter-Operator B-Galactosidase gene P O lacz Structural genes Permease gene lac Operon lacy Transacetylase gene lacA
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- In the presence of cephalosphorin, what will be the protein product of the prokaryotic cell gene shown below? 5'-CATGTCAGTAAGGCATCT-3' (Assume this is a segment of a larger structural gene that will be translated into protein in its entirety.)Q1. Predict the effects (on translation of coat gene and replicase gene) of the following mutations on phage R17 coat gene and replicase gene translation and explain the logic of your answers: a. An amber mutation (premature stop codon) six codons downstream of the coat gene initiation codon. b. Mutations in the stem loop around the coat gene initiation codon that weakens the base-pairing in the stem loop. c. Mutations in the interior of the replicase gene that cause it to base-pair with the coat gene initiation codon.1. Describe which enzymes are required for lactose and tryptophan metabolism inbacteria when lactose and tryptophan, respectively, are (a) present and (b)absent. 2. Contrast positive versus negative regulation of gene expression. Describe therole of the repressor in an inducible system and in a repressible system.
- Which of the following statements is/are true about gene regulation in bacteria? Choose all that could apply Multiple answer apply A genes with related functions are often grouped together and are transcribed into a single piece of RNA B small molecules act as sensors and bind DNA to change its 3D shape C enhancers are commonly used to regulate transcription D repressor proteins block transcription by binding to operator sequences E activator proteins bind near promoters and increase the efficiency of transcriptionYes or no only. rna seq can provide sequence and expression data do riboprobes synthesize bu in vitro transcription? does rna causes mutations and lose of function of specific genes?Brenner’s m mutant phages (m1–m6) described inFig. 8.8 were suppressed when grown in suppressor(su−) mutant bacteria; they produced full-length Mproteins that functioned like wild-type M protein.a. What gene do you think was mutant in the su−bacteria?b. When the m− phages were propagated in the su−bacterial strain, not all of the proteins made by themutant m alleles were identical to wild-type Mprotein. How did some of them differ?
- 1) Antibiotic-resistance is one of the gravest dangers to the world’s medical system. As a result, Dr. Jhon Peῆa decides to apply for a research grant from the NIH to investigate novel targets for antibiotic treatment in his lab. Most bacteria have developed resistance to counter the effects of the current crop of antibiotics, which largely target the cell wall, plasma membrane, or ribosomes. Dr. Peῆa’s team has instead been investigating bacterial transcriptional sites and all of his students are asked to attend a group Zoom meeting to discuss ideas to put into the grant. The lab technician, Jazmin, wonders if anyone thought of blocking the entrance way to the ribosome, the translator of mRNA? What does the rest of the lab think? a Considering the grant is for transcriptional targets, this makes no sense. b This makes perfect sense since the bacterial ribosome has a different structure than the eukaryotic ribosome, which everyone learns in Intro Bio in their…Locate a gene expression data set on Gene Expression Omnibus using "expression by hybridization" involving Rabies virus or related virus Use GEO2R to perform pairwise group analysis Explore and identify the most regulated genes: gene/protein functions of Rabies virusand Define the significance of these genes/proteins in the disease process.The following diagram describes the mRNA sequenceof part of the A gene and the beginning of the B geneof phage ϕX174. In this phage, some genes are read inoverlapping reading frames. For example, the code forthe A gene is used for part of the B gene, but the readingframe is displaced by one base. Shown here is the singlemRNA with the codons for proteins A and B indicated.aa# 5 6 7 8 9 10 11 12 13 14 15 16A AlaLysGluTrpAsnAsnSerLeuLysThrLysLeumRNA GCUAAAGAAUGGAACAACUCACUAAAAACCAAGCUGB MetGluGlnLeuThrLysAsnGlnAlaaa# 1 2 3 4 5 6 7 8 9Given the following amino acid (aa) changes, indicatethe base change that occurred in the mRNA and theconsequences for the other protein sequence.a. Asn at position 10 in protein A is changed to Tyr.b. Leu at position 12 in protein A is changed to Pro.c. Gln at position 8 in protein B is changed to Leu.d. The occurrence of overlapping reading frames isvery rare in nature. When it does occur, the extentof the overlap is…
- Matching type Choices are in the picture 6. RF1 and RF2 recognize the three bases to terminate the process 7. CAAT box is found 80 nucleotides from the actual site 8. rho factor and sequence of uracil in a loop conclude the process 9. sigma factor binds to RNA polymerase in the promoter region 10. methionine is removedWhich of the following would best ensure that a kidney cell and a liver cell from the same person would express different genes? Group of answer choices differences in introns unique sets of transcription factors they only have specific genes unique sets of promoters 2. Refer to the metabolic pathway illustrated. If A, B, and C are all required for growth, a strain mutant for the gene encoding enzyme A would be able to grow on medium supplemented with which of the following nutrient(s)?The gene encoding the beta subunit of RNA polymerase fromEscherichia coli is said to be orthologous to the rpoB gene ofBacillus subtilis. What does that mean about the relationshipbetween the two genes? What protein do you suppose therpoB gene of B. subtilis encodes? The genes for the differentsigma factors of E. coli are paralogous. What does that sayabout the relationship among these genes?