How would one go about identifying the active site residues in the laboratory?
Q: How does zinc monitor some of the enzyme structures?
A: Metalloenzymes are those which contain metal ion at the active site. The metal ion at the active…
Q: In 1828, Wöhler was the first person to synthesize an organic compound (urea, from ammonium…
A: Friedrich Wohler carried out several reactions that resulted in the production of Urea [NH2)2CO], an…
Q: Describe several general ways that Ab-Ag reactions are detected.
A: Antigens are the protein molecules capable of inducing an immune response. Antibodies…
Q: Given the active site and reaction mechanism below, what is the mechanism of rreversible inhibition…
A: The mechanism followed by the given example is of Transition state analog Chemical compounds with a…
Q: Can you explain in detail why do cells use general acid catalysis and not specific acid catalysis?
A: Cellular metabolism involves many pathways like glycolysis, Krebs cycle, lipolysis and energy…
Q: Given the active site diagram below, which best described the mechanism(s) of Active Site `NH2. SH…
A: Acid-Base Catalysis: Here chemical reaction is catalyzed either by an acid or a base. Acid-Base…
Q: Are the amino acid residues at the active site need to be in close proximity to one another…
A: Enzymes are the catalysts that catalyze biochemical reactions. These are proteinaceous in nature as…
Q: What are the modern techniques used to identify the active sites of an enzyme?
A: The part of the enzyme where the substrate binds is called the active site. An enzyme molecule has…
Q: What is the difference between an allosteric site and an activesite?
A: Allosteric and active sites are present in an enzyme. Enzyme is a catalytic molecule that increases…
Q: Given the active site diagram below, identify the numbered region corresponding to an acidic…
A: An acid is a molecule that can accept a proton from the solution and a base is a molecule that can…
Q: How different biochemical material is sterilized? What are its importance in biochemical industry?
A: A chemical substance whose occurrence can be traced in living organisms is referred to as a…
Q: Given the active site and reaction mechanism below, what is the mechanism of irreversible inhibition…
A: The given example is uncompetitive inhibition. Uncompetitive inhibition, also known as…
Q: How does competitive inhibition differ from non-competitive inhibition?
A: COMPETITIVE inhibition occurs when the inhibitor and substrate both attach to the enzyme's active…
Q: Is It Possible to Design an Enzyme to CatalyzeAny Desired Reaction?
A: Enzymes catalyze thermodynamically favorable reactions causing them to proceed at extraordinarily…
Q: How can you distinguish between a competitive inhibitor and an uncompetitive inhibitor…
A: Competitive inhibitor- In competitive inhibitor, an inhibitor that resembles the normal substrate…
Q: Why is the regulation of biochemical processes important?List three reasons
A: The reactions which take place in living beings are called biochemical processes. The regulation of…
Q: From the active site diagram below, please indicate the cofactor from the indicated components. HO 4…
A: Cofactors are the non protein molecules that help the enzyme to carry out catalysis. Enzymes without…
Q: Why we use glutaraldehyde in production of biomaterials ? What can we use instead of glutaraldehyde?
A: In the field of biomaterials, there is a combination of medicine, physics, biology, and chemistry.…
Q: Why are buffers important in biochemical experiments?
A: Buffer : solution which resists any pH change via the addition of any acidic, or the basic…
Q: Why is it unlikely that nonenzymatic catalysts operate by preferentially binding the transition…
A: Both enzymes and catalysts control the speed of a reaction. The distinction between enzymes and…
Q: what is a cofactor
A: The enzyme sometimes need co-factor to work. These co-factor help enzyme to slow down or speed up…
Q: How has the analysis of enzyme kinetics been fundamental to the modern design of pharmaceuticals?
A: Drug development occurs on the basis of reactivity of the target cell or organs in response to a…
Q: What are cooperativity, coenzyme and isoelectric point? Briefly explain.
A: Enzyme is basically biocatalyst that increase the rate of chemical reaction without itself being…
Q: How can inhibitor binding to an enzyme be quantified?
A: Inhibitor binding is competitive with the enzyme when the substrate is also present in the sample.…
Q: What is meant by general acid catalysis with respect to enzyme mechanisms?
A: An enzyme draws substrates to its active site, catalyzes the chemical reaction that creates…
Q: what are the clinical implications of measuring alkaline phosphatase isomers and their activity ?
A: Alkaline phosphatase isomers are a group of isoenzymes with low substrate specificity that catalyzes…
Q: What is the difference between pure and mixed noncompetitive inhibition?
A: Pure competitive inhibition is the type of inhibition in which the inhibitor binds to the site other…
Q: Can you explain the biosynthetic reaction (efflux of intermediates)?
A: Biosynthetic reactions are those reactions in which enzymes catalyse the process of conversion of…
Q: Is the formation of a serine ester an example of acid/base catalysis, group transfer or…
A: Serine residue has an -OH group acting as a nucleophile, attacking the carbonyl carbon of the…
Q: Must the amino acid residues in the active site be near each other along the polypeptide chain?…
A: The polypeptide chain is considered as the long sequence of amino acids, which exhibits amino acid…
Q: DIY Glue State the materials that use to make it. Show the mechanisms of the reaction that occur.
A: The materials necessary for the preparation of glue are as follows: 12 cup of skim (non fat) milk 1…
Q: What is the biochemical role of folic acid?
A: Folic acid is also named as vitamin B9, which helps in the synthesis of genetic material. It helps…
Q: What does a cofactor do?
A: A biomolecule that is not a protein is referred to as a cofactor. Any metal ion or organic molecule…
Q: Given the active site diagram below, identify the acidic residue from the indicated components.
A: Amino acids are basic unit for any protein or polypeptide chain. It consist of -COOH, -NH2, side…
Q: How do you think the appropriate enzyme would have affected the reaction (degree of completion,…
A: Stereochemistry : It is the study of how molecules are affected by the way their atoms are arranged…
Q: What Can We Learn About Biochemical Events From Thermodynamic Parameters?
A: There are many metrics that may provide information on biochemical processes; nevertheless, a single…
Q: How does stereoisomerism features affect the pharmacodynamic effect? explain briefly
A: To study a biological property and physical property of an organic compound to become a drug is…
Q: What is the spatial relationship of the critical amino acid residues in the active site?
A: Amino acids are small biomolecules that exist naturally in a zwitterion state where the carboxylic…
Q: Name the components a bioreactor must possess to achieve the desired product?
A: Bioreactor is defined as a vessel that carries out a biological reaction. It is used to culture…
Q: How can the same biochemical be both a reactant (a starting material) and a product?
A: In chemical and biochemical reactions, a reactant is the starting compound that can be converted…
Q: How can competitive and pure noncompetitive inhibition be distinguished in terms of KM?
A: Km is also known as Michaelis constant. It is one of the parameters to determine enzyme activity.…
Q: For a weak ester drug that is unstable in highly acidic or alkaline solutions, what strategies could…
A: Bioavailability relates to the ability and rate of the active ingredient (drug or metabolite) that…
Q: What are biogenic amines? What role do they play in the body? Give exact examples of biogenic amines…
A: Biogenic amines are the substances which plays many important role in functioning of our body.
Q: What are the functions of the sterols in human body?
A: Asked : Functions of sterol in human body
Q: Explain how would you go about identifying the active site residues in the laboratory
A: Active site is defined as the enzyme region where substrates bind and lead a reaction. The active…
Q: what is the properties of an enzyme that make it a good biological catalyst?
A: Enzymes are organic substances (mostly proteins) that accelerate or alter the rate of chemical…
Q: What is Biogenic Amines?
A: Amino acids are the monomers of proteins.
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- Why are buffers important in biochemical experiments?In 1828, Wöhler was the first person to synthesize an organic compound (urea, from ammonium cyanate). Howdid this contribute, ultimately, to biochemistry?What is the classical method to check the quality of Nucleic Acid Product? Discuss its significance