Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
This lab will introduce the technique of streak plate isolation, which is a method used to isolate a single bacterial colony from a culture. This technique is used to identify a particular bacteria and can be used to determine the type of bacteria present in a sample. The lab will involve obtaining a sterile inoculating loop, streak plate, and a culture of bacteria. The loop will be used to draw lines on the surface of the streak plate which will be used to spread the bacteria. The plate will then be incubated for 24-48 hours at a temperature that is appropriate for the bacteria before observing for bacterial growth and isolating a single colony.
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