In doing a phytochemical determination of natural products, one of the tests involved the determination of indoles. Would the presence of plant secondary metabolites interfere with the tests for proteins giving false-positive results? Why or why not?
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- In isolating metabolites and testing for phytochemicals, why is it recommended to dry the leaves in open air and not in direct sunlight? Are there other ways to do dry the leaves?In the isolation of metabolites and tests for phytochemicals, why should people dry the leaves in open air and not in direct sunlight? Could there be other ways to do dry the leaves?The three forms of the anthocvanins in red cab.-bage extract are red, blue, and yellow. How do you account for the violet and green colors you observed in some of vour color standards?
- The most probably number (MPN) method for measuring Nitrifiers in soil Microbiology ?Answer the following questions. Why is 80% ethyl alcohol is considered as the solvent of choice during phytochemical screening? What are the different types of extraction? Differentiate each. Differentiate Primary versus Secondary Metabolites and give examples. How are flowers, fruits and seeds dried? Explain why flowers requires greater care in drying.What other characteristics should a plant species have to be a good candidate for use in making cellulosic biofuel?
- What would be the main consideration in the media composition for growth-linked products vs products Which are not directly linked to the growth?What is the chemical composition of pure cane sugar, equal original and splenda? and explain based on the composition the sweetness of different sugars and artificial sweeteners.What is a nutrient solution? Why is it more convenient to use liquid medium, as opposed to soil, to grow plants in nutritional studies?
- Which of the following is considered as an appropriate plant extraction technique? 1. Thermolabile components of plants are best extracted by reflux2. Concentration and drying of extracts in vacuo are necessary to ensure the stability of components3. Polar bioactive components should be extracted from the plan matrix using petroleum ether4. Extraction should always utilize an equal solvent-sample ratio regardless of the absorbing nature of the solvent matrix!What initiatives could be taken if plant tissue cultures are suffering severely due to alkaloid production and accumulation in the medium.You want to prepare 500 mL of the macronutrient and micronutrient stock solutions which have 100X and 1000X higher concentration, respectively, than the required concentration in the culture mediuma)Approximately 4 % (w/v) sucrose and 0.8 % (w/v) agar are added into the medium.Determine the amount of sucrose and agar (in gram) required in order to prepare 200 mL MS medium. Show your work