In this qPCR plot, which sample had the least abundant starting amount of DNA?

Q: In pcr experiment, Does electrophoresis show that only DNA products of the desired size are present?…

A: A DNA sequence may be amplified trillions of times via PCR, yielding enough DNA copies for further…

Q: What is the functional difference between regular PCR and qPCCR?

A: PCR stands for a polymerase chain reaction. As the name suggests that polymerase chain reaction…

Q: What are STRs, and why are they useful for DNA profiling?

A: Introduction  In the genome there are usually two type of nucleic acid sequence present. One is Non…

Q: How Short hybridization probes can distinguish single-base mismatches. ?

A: Hybridization probes are the fragments of RNA or DNA molecules that have variable lengths and are…

Q: If a pair of 10-mer primers are used in a PCR, what would be the number of expected binding sites in…

A: PCR is a polymerase chain reaction, in this process DNA is amplified . For this amplification,…

Q: Which is the correct order of the PCR reaction?

A: Introduction PCR or the polymerase Chain Reaction is a molecular technique used to amplify a…

Q: How to isolate cpDNA for whole chloroplast DNA sequencing (MiSeq system)? Any universal primer or…

A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…

Q: diiferentiate  between PCR and Sanger sequencing?

A: PCR (polymerase chain reaction is a technique through which a DNA segment is copied to million folds…

Q: Explain the principles of DNA migration of sample A in the electrophoresis experiment

A: Electrophoresis is a separation technique that was used to separate the mixture of molecules from a…

Q: What does the alcohol do in this experiment? Why does the DNA rise to the top after adding alcohol?…

A: Extraction of DNA from fruits.

Q: Explain how agarose electrophoresis separates DNA fragments. Why does a smaller DNA fragment move…

A: Electrophoresis the separation of fragment based on their size and charge.Agarose gel…

Q: What is the function of the homogenizing solution in DNA isolation? What are its components?

A: In the process of DNA isolation it is very important to breakdown or lyse the cell this allow the…

Q: What is Sanger sequencing used for?

A: DNA sequencing is defined as a process in which DNA’s nucleotide sequence is determined. The main…

Q: How does gel electrophoresis separate different-sized DNA fragments?

A: Gel electrophoresis is a molecular biology technique used to separate macromolecules like DNA, RNA…

Q: Why is the annealing temperature different between samples during DNA extraction & marker gene…

A: Introduction Polymerase chain reaction or PCR  laboratort technique by which millions to billions of…

Q: If it is not possible to have three bands for one STR region when looking at the DNA from one…

A: The hereditary unit of an organism is DNA (deoxyribonucleic acid). Individuals' DNA is made up of a…

Q: If it is not possible to have three bands for one STR region when looking at the DNA from one…

A: DNA (deoxyribonucleic acid) is the hereditary unit of an organism. The DNA consists of a specific…

Q: What is the relevance of the sample size to the choice of test organisms for genetic studies?

A: The sample size is the number of organisms chosen for a study or experiment. It can be large or…

Q: Using the table below, what volume from the master mix will be dispensed to each sample tube if the…

A: Answer:  As we know, C1V1= C2V2, where C1 and C2 = initial and final concentrations V1 and V2 =…

Q: Assuming a circular piece of DNA (plasmid) was used as starting material, how many restriction sites…

A: Restriction enzymes are specific endonuclease that can cut a DNA at a particular sequence.…

Q: Define the Single-cell sequencing (SCS) ?

A: Working of Single-cell deoxyribonucleic acid genome sequencing involves analytic one cell,…

Q: How does gel electrophoresis separate DNA fragments of your unknown sample and How will you be able…

A:

Q: What is the principle behind to Sanger sequencing?

A: Sanger sequencing is a method of DNA sequencing, which is based on selective incorporation of…

Q: If the cloning was successful and the plasmid contained the elements needed for blue-white…

A: The screening of transformants after the cloning is a critical step as it allows the identification…

Q: What is the function of the chaotropic salt in DNA isolation?

A: These salts are strong protein denaturing agents.

Q: Approximately how many base pairs were identified in the human genome working drafts?

A: The human genome project is a scientific project led by James Watson and Francis Collin, started in…

Q: What is the cause of an extra band in a DNA ladder in agarose gel electrophoresis?

A: The following can lead to the formation of an extra band in the DNA ladder lane - 1. Contamination -…

Q: In this picture of gel electrophoresis of DNA, which sample contains the longest/largest piece of…

A: DNA (Deoxyribonucleic acid) is a double helical structure that consists of nucleotide. Nucleotide…

Q: What are the consequences for a DNA sequencing reaction if the ratio of dideoxyribonucleoside…

A: Introduction  DNA is composed of nucleotides arranged in a specific sequence. Prior knowledge of DNA…

Q: Based on the electrophoresis experiment, 0.7% agarose gel concentration was used. If the gel…

A: Agrose gel is a polysaccharide extracted from see weed. It is extracted as a linear structure. But…

Q: In an in situ hybridization experiment, what is the relationship betweenthe base sequence of the…

A: Hybridization refers to the pairing or bonding of two different molecules.

Q: When is low diversity especially problematic in sequencing?

A: In sequencing micro RNA any method which requires use of in house barcodes may results in giving low…

Q: Short hybridization probes can distinguish single-base mismatches?

A: Hybridization probes are the fragments of RNA or DNA molecules that have variable lengths and are…

Q: If you were to isolate genomic DNA from two people and perform restriction enzyme digestion followed…

A: Agarose gel electrophoresis is a technique for distinguishing DNA bands based on size. To…

Q: What key structural features of the DNA molecule underlie its ability to be faithfully replicated?

A: DNA is known as deoxyribonucleic acid. DNA is the genetic material in humans and other organisms.…

Q: What will be the result if we resequence the same samples contributed to the reference genome and…

A: Many hereditary disorders may now be tested for through "genetic testing". Some diseases,…

Q: Is a faded gel matrix in a DNA ladder in agarose gel electrophoresis still useable for analysis or…

A: Agarose gel electrophoresis is a technique to separate different fragments of DNA on the basis of…

Q: Gel Electrophoresis Separates DNAFragments According to which thing?

A: Electrophoresis is a technique used for separating the components of a mixture of charged molecules…

Q: When is the transformation factor used?

A: According to Griffith's experiments, based on the bacterial transformation through the Streptococcus…

Q: During PCR amplification in preparation for DNA sequencing, why were there different colors at the…

A: Polymerase chain reaction is a process by which millions of copies of a specific DNA segment are…

Q: how Inverse PCR identifies genes withtransposon insertions?

A: Polymerase chain reaction (PCR)is the molecular technique that is used in the amplification of…

Q: What are the steps in extracting DNA from a Banana using simple household materials like dishwashing…

A: DNA extraction from banana is most commonly followed as banana is triploid with three sets of…

Q: What information do learn from the results of the DNA extraction?

A: Hi! Since you posted more than one question, we will be answering one for you. If you want the other…

Q: (For PCR reactions) If a main amplicon for allele 10 is 444 bp in length. How long in base pairs is…

A: the amplicon size should be shorter so that the extension time is within the range of PCR cycle

Q: Among all these primer pairs from Primer-Blast, which is the best to use for a PCR reaction and why?

A: The hereditary currency of an organism is the gene. The gene is present in the DNA of an organism.…

Q: What are microcarrier and macrocarrier in Gene gun method.

A: Biolistic bombartment (gene gun) is one of the direct gene transfer methods. It physically shoot the…

Q: What is the rationale for the salting out technique for DNA extraction?

A: DNA is the genetic material that stores all the genetic information. Extraction of DNA is applied…

Q: What are the different methods of quantifying DNA? How would you determine if your

A: Quantitation of DNA ,methods used for quantifying DNA ---* Quantifying of DNA -- In various methods…