Laboratory Value Hemoglobin (Hg) Hematocrit (Hct) Description of Laboratory Value- What does it tell us? Platelets Red blood cells White blood cells Mean corpuscular volume (MCV)
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- Composition of Blood 1. Determine the physical characteristics of plasma after it centrifuge for 5 minutes? 2. Differentiate the plasma from serum after letting it stand for 15 minutes and then putting it into centrifuge for 5 minutes 3. Microscopic Evaluation of Formed Elements of Blood - Describe the appearance of the erythrocytes, leukocytes and their different types, and the platelets - Discuss the functions of each of the formed elements in the blood and give their relative percentages.True or false Chemically-linked hemoglobin moelcules can stay in the blood for as long as 72 hrs. Cow hemoglobin can be safelty used by humans. Free hemoglobin produces vasoconstruction of blood vesselsand increases blood pressure in patients.A. Calculate the final percent concentration of the erythrocytes in each well: (NaCl, CaCl2, MgCl2) each solution has a concentration of 0.25M erythrocyte suspension solution (1mL of erythrocyte solution containing 10% washed red blood cells.) 135 microliters of each solution were added to individual wells + 15uL of the erythrocyte solution ex: Well #1= 135uL of (0.25M CaCl2 + 15uL erythrocyte sol.) B. What would be the final concentration of a well with 135uL (0.125M NaCl) + 15 uL erythrocyte solution?
- Calculate hematocrit for Susie's whole blood sample, given the following information: RBC volume = 44 ul, White blood cells and platelets = 3 ul, and Plasma = 54 ul. 81.48% 43.56% 12.27% 44.89%Give the relative abundance of the nine different cells (as mentioned below) in one cubic centimeter of blood sample. 1. The human erythrocytes 2. Granulocytes a. Neutrophil b.Eosinophil c.Basophils 3. Agranulocytes a.Lymphocytes b. Monocytes c.platelets d. frog erythrocytes e. Frog thrombocytesWith the appearance of strong oxidizers in blood in erythrocytes one of the following substances is formed first of all: 1. carbhemoglobin 2. carboxyhemoglobin 3. oxyhemoglobin 4. methemoglobin 5. deoxyhemoglobin
- Calculate the hemoglobin concentration of an unknown sample given the following info: concentration of known = 12.5 g/dl; absorbance of unknown = 0.48; absorbance of known = 0.65 a. 16.9 g/dl b. 9.2 g/dl c. 8.7 g/dl d. 11.3 g/dl e. 10.4 g/dlWhy is copper sulfate considered an obsolete method of hemoglobin determination? What are the layers of centrifuged blood? Draw and label What is trapped plasma? Discuss What factors contribute to increased and decreased Eythrocyte Sedimentation RateWhich of the following hemolysate preparation steps is not correct? A. Erythrocyte hemolysate contains various glycolysis enzymes, hemoglobin molecules, various coenzymes B. The packed erythrocyte is washed 4 times with ‰ 9 NaCl. C. The blood collected in anticoagulant tubes is centrifuged at 3000 rpm for 15 minutes to separate the plasma. D. Erythrocytes are hemolyzed under hypertonic conditions.
- Which of the following factors play a role in theoxygen–hemoglobin saturation/dissociation curve?a. temperatureb. pHc. BPGd. all of the aboveAmong the given statements. which ones are true for hemoglobin? Select the correct response(S). It has ferrous cation that is coordinated by the electrophilic N atoms of the porphyrin ring. It has 4 sub-units with each sub-unit binding an oxygen molecule with the same efficiency. It has 4 sub-units with each sub-unit binding an oxygen molecule in an increasing efficiency. It has ferric cation that is coordinated by the electrophilic N atoms of the porphyrin ring. It has 4 sub-units with each sub-unit binding an oxygen molecule in a decreasing efficiency. It has ferric cation that is coordinated by the nucleophilic N atoms of the porphyrin ring. It has 4 sub-units with each sub-unit binding an oxygen molecule in a fluctuating efficiency.It has ferrous cation that is coordinated by the nucleophilic N atoms of the porphyrin ring.Background: The hematocrit of whole blood is the percent of the blood (by volume) that is made up of cells. For example, if a person has a hematocrit of 40, it means that 40% of the blood volume is cells and the rest is plasma. The average hematocrit of a normal human male is 42 and 38 for a normal human female. The degree of bodily activity, anaemia, as well as altitude, are among some factors that affect hematocrits. calculation to get the % red blood cells (RBC): RBC present over total liquid x 100 The tubes in the image were all centrifuged at maximum speed for 5 minutes. Describe your observations of the hypo-, iso-, and hypertonic hematocrit tubes. Also calculate the % volume of red blood cells in each tube.
