10. Modify the diagrams below to reflect the activation and repression of lac operon. Usearrows, lines, X's, etc. to illustrate control of gene expression.Sa. ActivationlactoselactoseCAPlac Irepressorallolactoseb. RepressionCAPallolactoselac IrepressorCAMPсCAMPсpromoter operator+1RNAPol+1RNAPolpromoter operatorlac Zlac Zlac Ylac Ylac Alac ADNADNA

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Description: 10. Modify the diagrams below to reflect the activation and repression of lac operon. Usearrows, lines, X's, etc. to illustrate control of gene expression.Sa. ActivationlactoselactoseCAPlac Irepressorallolactoseb. RepressionCAPallolactoselac Irepres

Lac operon consists of the cluster of genes. The letters depicted in the lac operon below are as…

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lactose a. Activation CAP lac I repressor allolactose b. Repression CAP lac I repressor CAMP с CAMP с RNA Pol promoter operator RNA Pol promoter operator +1 lac Z lac Z lac Y lac Y lac A lac A DNA DN.

lactose a. Activation CAP lac I repressor allolactose b. Repression CAP lac I repressor CAMP с CAMP с RNA Pol promoter operator RNA Pol promoter operator +1 lac Z lac Z lac Y lac Y lac A lac A DNA DN.

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter16: Gene Expression

Section: Chapter Questions

Problem 9RQ: The a/a operon is an inducible operon that controls the production of the sugar arabinose. When...

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The a/a operon is an inducible operon that controls the production of the sugar arabinose. When arabinose is present in a bacterium it binds to the protein AraC, and the complex binds to the initiator site to promote transcription. In this scenario, AraC is a(n). activator inducer repressor operator
1) A. What is the difference between a repressible vs, an inducible operon? B. Using diagrams and words, describe how the Lac Operon is regulated by different levels of lactose and glucose. Be sure to show what’s happening at the molecular level, including the roles of lactose, glucose the repressor protein, cAMP and CAP.
1. What is the default state of the lac operon? a. LacI repressor is sitting on the operator sequence and blocks RNAP from transcribing lacZYA. b. LacI repressor is not in a correct shape to bind to the operator sequence. c. LacI repressor is not produced in the absence of lactose. d. Allolactose is sitting on the operator sequence and blocks RNAP from transcribing lacZYA.
. In an effort to determine the location of an operator sitefor a negatively regulated gene, you have made a seriesof deletions within the regulatory region. The extent ofeach deletion is shown by the line underneath the sequence, and the resulting expression from the operon(i = inducible; c = constitutive; − = no expression) isalso indicated.... GGAT C T T AGCCGGCTAACATGATAAATATAA......C C T AGAATCGGCCGA TTGTA C T A TTT ATAT T ...1 i2 –3 c4 –5 ca. What can you conclude from these data about thelocation of the operator site?b. Why do you think deletions 2 and 4 show no expression of the gene?
Select all of the cis-acting regulatory elements of the lac operon. Operator CAP+cAMP CAP+cAMP binding site LacZ
Choose correct option and do explain plz. 1. The protein complex that helps RNA polymerase to cross nucleosomes during extension is:a. SWI-SNFb. poly A polymerasac. TFIISd. FACT 2. The polydenylation is carried out by:a. primaseb. polymers poly ac. a reverse transcriptased. adenylyltransferase 3. The lactose operon produces a polycistronic mRNA that includes four genes: Lacl, LacZ, LacY and LacA. True or false?
Describe the main functional differences between the TRP and the Lac operons. Main functional differences DO NOT refer to the fact that one operon involves TRP and the other involves Lac. They also don’t include that there are different genes found in the TRP operon compared to the Lac operon or that the repressor in the Lac operon is called Lac l. Also DO NOT simply list how both operons work to answer this question.
1) Assume you have an operon that is repressible. In this case, the rate of synthesis of repressor protein must depend on the level of a) co-repressor b) inducer c) both d) neither
1. Assuming that the level of glucose is low, a mutation in the repressor associated with the lac operon of E. coli which prevents binding of the repressor to lactose should result in: a. constitutive expression of the lac operon genes b. lack of expression or refuced expression of the lac operon genes under all circumstances c. expression of the genes only when lactose is present d. expression of the genes only when lactose is absent e. lack of expression of the lac operon genes, resulting in an increase in lactose production
Strain ROFL4 has a premature stop mutation in the lacZ gene, resulting in a nonfunctional b-galactosidase. Otherwise all other parts of the operon are functional. Circle the least number of components for an F' plasmid that will restore normal regulation and function of the lac operon in the resulting partial diploid. (may need more than one) (a) lacI+ (b) lacO+ (c) lacP+ (d) lacZ+ (e) None, cannot be restored.
The figure at the beginning of this chapter showsthe binding of both a Lac repressor tetramer and aCRP-cAMP complex to the regulatory region of thelac operon.a. What is the key feature of a regulatory protein suchas the Lac repressor or CRP that allows it to regulate specifically the genes or operons it is supposedto control?b. On the figure, show the positions of the followingcomponents: (i) A Lac repressor monomer; (ii) a Lacrepressor dimer; (iii) all four DNA binding domains ofthe Lac repressor tetramer; (iv) a single helix-turnhelix motif; (v) the o1 part and either the o2 or o3 partsof the lac operator (assume the operon would be transcribed from right to left on the figure); (vi) themultimerization domains of the four Lac repressormonomers; (vii) an inducer-interacting domain; (viii)the CRP-cAMP complex; and (ix) a DNA loop.c. What is the physical basis for the formation of theDNA loop shown in the figure?d. On the figure, show the position of two axes ofsymmetry in the sequence…
1. If the above gene is one of the three structural genes of the lac operon that codes for theprotein/ enzyme responsible for breaking lactose into two molecules of simple sugars,a.) what triggers the activation of this gene?b.) what triggers the inactivation of this gene?c.) what substance is attached to the operator region of the operon in the absence ofactivator?d.) what gene is responsible for the synthesis of the substance used to attach in theoperator region in the absence of activator? e.) what substance is attached to the promoter region of the operon in this case?