Many proteins inside cells are regulated by phosphorylation and dephosphorylation. Imagine a metabolic reaction for which the enzyme is completely active (ON) when not phosphorylated and completely inactive when phosphorylated (OFF). 0 Enz A kinase phosphatase Enz A PO4-3 ON OFF A. What molecule is typically the source of the phosphate group that is transferred to this enzyme by the kinase? B. Inside the cell, the rate of the reaction catalyzed by this enzyme can vary continuously between very fast and very slow (or zero). How is it that the reaction in the cell can vary in this way, whereas an individual enzyme molecule is either ON or OFF?

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Many proteins inside cells are regulated by phosphorylation and dephosphorylation. Imagine a
metabolic reaction for which the enzyme is completely active (ON) when not phosphorylated and
completely inactive when phosphorylated (OFF).
-PO4-3
0
Enz
A
kinase
phosphatase
Enz
A
OFF
ON
A. What molecule is typically the source of the phosphate group that is transferred to this enzyme
by the kinase?
B. Inside the cell, the rate of the reaction catalyzed by this enzyme can vary continuously
between very fast and very slow (or zero). How is it that the reaction in the cell can vary in this
way, whereas an individual enzyme molecule is either ON or OFF?
Transcribed Image Text:Many proteins inside cells are regulated by phosphorylation and dephosphorylation. Imagine a metabolic reaction for which the enzyme is completely active (ON) when not phosphorylated and completely inactive when phosphorylated (OFF). -PO4-3 0 Enz A kinase phosphatase Enz A OFF ON A. What molecule is typically the source of the phosphate group that is transferred to this enzyme by the kinase? B. Inside the cell, the rate of the reaction catalyzed by this enzyme can vary continuously between very fast and very slow (or zero). How is it that the reaction in the cell can vary in this way, whereas an individual enzyme molecule is either ON or OFF?
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