Mary wanted to know the amount of microbes in her garden. To do this, she took a 10g soil sample, added urea, and then let it incubate for one week. She then performed serial dilutio shown below:
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- A sample of Earth Alive Soil Activator was diluted by transferring 1 gram into a 9 ml dilution blank (tube A) and then serially diluted five (5) more times by transferring 1 ml of a previous dilution into a tube of 9 ml of sterile water. After making these serial dilutions, 2 ml samples were taken from tubes D, E, and F, added into empty petri dishes, and molten PCA was poured into each petri dish. Tubes A, B, and C were heated in a 80˚C water bath for 30 minutes, 2 ml samples of these tubes were added into empty petri dishes, and molten PCA was poured into each petri dish. These plates were allowed to solidify, and they were incubated at 35˚C for 48 hours. After the incubation period, you counted CFUs on all 6 plates. The results of your CFU counts are contained in table 1. Table 1. CFU counts for 3 plates from the heated dilutions (A-C) and the 3 plates from the non-heated dilutions (D-F) # CFUs Not heated Heated A -- 400 B -- 45 C -- 6 D 350 -- E 31 -- F 2 --…Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet 11. Bacteriologic filters (Seitz, Chamberlain, Berkfield) 12. Petri dish 13. Culture tubes 14. Hanging drop slide 15. Durham’s tubeDisinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%
- thirty-six colonies grew on nutrient agar plates when 1.0ml of a 1/10 dilution of a water sample was plated using the standard plate count methodology. How many bacteria were in the original water sample? a.4/ml b.36000/ml c.360/ml d.36/ml e.3600/mlIf a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium cannot ferment mannitol.match coloumn a to coloumn b coloumn a coloumn b 11. Safe drinking water is totally free from chemicals.12. Total bacteria present in water samples from water vending machines isdetermined using an enzyme-substrate test. 13. All water samples from the La Trinidad water district must report <1.1 MPNper 100 mL total coliform to comply with DOH standards. FALSE All water samplesfrom the La Trinidad water district must report <1.1 MPN per 100 mLthermotolerant coliform to comply with DOH standards.14. Baguio ice plant and cold storage samples must be tested with the presenceof total bacteria, total coliform, and thermotolerant coliform once a month.15. A water sample that contains beyond the maximum acceptable level oftotal coliform must be retested. a. true b. false
- Mary Jo prepared a series of diluted samples by taking 1 mL of sample and adding it to 4 mLs of diluent. She then diluted the sample further by adding 1 mL of diluted sample to 19 mLs of diluent. What is the final dilution factor ?Describe the microbial growth on the plates of the agar surface swabbed with sample from the surface of your interest (doorknob).Match the following terms with the best description. Question 6 options: A purified substance of a known compostion. The process of monitoring accuracy & reproducibility of known control results. How close the test results are when repeated. 1. Standards 2. Precision 3. Quality Control