See attached, you have a table that is perhaps required for some of the questions. Answer question number 7 in your own words. Do not copy and paste from google. Lab 5: EnzymesResults:Table 1, Effect of enzyme concentrationAmount of lodine Test Results (color)AmylaseEnzyme0 drops3 drops6 drops9 dropsBoiling waterbath37° waterbathIcebath0 minwaterdark blueTable 2. Effect of temperatureTemperaturedark bluedark bluedark bluelodine TestResults(color)10 mindark blueyellowBrown5 mindark bluedark brownbrownlight brown56710 min9dark blueTable 3. Effect of pHpHlight brownlight greengreen5 minyellowyellowyellowBenedict'sTest Results(color)lodine TestResults (color)yellow10 minbright bluelight blueyellowyellow/redlodine TestResults (color)15 minyellowyellowyellowfaint yellow Part 35. What was the optimal temperature for amylase? Explain how your iodine testresults support your answer.56. Why did the enzyme activity differ in the ice bath compared to the boiling waterbath?Part 47. What was the optimal pH for amylase? Explain how your iodine test resultssupport your answer.8. Pepsin is an enzyme that hydrolyzes proteins in the stomach.a. The pH in the stomach is 2. What do you think the optimum pH ofpepsin would be?b. What happens to the activity of pepsin when it enters the small intestinewhere the pH is 8?

In the given experiment we are using iodine test to test the enzyme activity of amylase. Amylase is…

Answered: Part 4 7. What was the optimal pH for…

Science

Biochemistry

Part 4 7. What was the optimal pH for amylase? Explain how your iodine test results support your answer.

Nutritional Sciences: From Fundamentals to Food, Enhanced Edition

3rd Edition

ISBN:9781337486415

Author:McGuire

Publisher:McGuire

Chapter2: Nutritional Assessment And Dietary Planning

Section: Chapter Questions

Problem II3DWPA

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Question

See attached, you have a table that is perhaps required for some of the questions.
Answer question number 7 in your own words. Do not copy and paste from google.

Lab 5: Enzymes
Results:
Table 1, Effect of enzyme concentration
Amount of lodine Test Results (color)
Amylase
Enzyme
0 drops
3 drops
6 drops
9 drops
Boiling water
bath
37° water
bath
Ice
bath
0 min
water
dark blue
Table 2. Effect of temperature
Temperature
dark blue
dark blue
dark blue
lodine Test
Results
(color)
10 min
dark blue
yellow
Brown
5 min
dark blue
dark brown
brown
light brown
5
6
7
10 min
9
dark blue
Table 3. Effect of pH
pH
light brown
light green
green
5 min
yellow
yellow
yellow
Benedict's
Test Results
(color)
lodine Test
Results (color)
yellow
10 min
bright blue
light blue
yellow
yellow/red
lodine Test
Results (color)
15 min
yellow
yellow
yellow
faint yellow

Part 3
5. What was the optimal temperature for amylase? Explain how your iodine test
results support your answer.
5
6. Why did the enzyme activity differ in the ice bath compared to the boiling water
bath?
Part 4
7. What was the optimal pH for amylase? Explain how your iodine test results
support your answer.
8. Pepsin is an enzyme that hydrolyzes proteins in the stomach.
a. The pH in the stomach is 2. What do you think the optimum pH of
pepsin would be?
b. What happens to the activity of pepsin when it enters the small intestine
where the pH is 8?

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