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PART B Re-watch the video and explain why the following steps are necessary: • Why use tweezers to put disks on the plate? • Why sterilize tweezers after each disk is put on the agar? Why spread the bacteria out all over the agar plate? • • Why put a disk with NO antibiotic on each agar plate? Why put a disk with newly purchased, fresh antibiotic on each plate? • Why put so many disks on each plate? • Why wait 16-20 hours after you have put the bacteria and antibiotic disks on the plate?

PART B Re-watch the video and explain why the following steps are necessary: • Why use tweezers to put disks on the plate? • Why sterilize tweezers after each disk is put on the agar? Why spread the bacteria out all over the agar plate? • • Why put a disk with NO antibiotic on each agar plate? Why put a disk with newly purchased, fresh antibiotic on each plate? • Why put so many disks on each plate? • Why wait 16-20 hours after you have put the bacteria and antibiotic disks on the plate?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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I need help with part B

Learning Task 16-03 - Antibiotic Effectiveness PART A • Record the steps that the laboratory technician follows in the video. • Highlight, by colour or bolding, the steps that ensure the experiment is sterile. Materials You will need: 6 millimeters filter paper disks, sterile Petri dishes, agar plates and no added antibiotics, sensitive and resistant bacterial cultures, an antibiotic to test and antibiotics control Steps 1. Pick colony and grow overnight 2. Dilute culture and grow to OD = 0.5 3. Prepare antibiotic stock 4. Add antibiotic to each disc 5. Dry discs at room temperature 6. Spread bacteria evenly on agar plate Place discs in center of sectioned areas 7. 8. Incubate plates 16-20h and measure zones PART B Re-watch the video and explain why the following steps are necessary: • Why use tweezers to put disks on the plate? • Why sterilize tweezers after each disk is put on the agar? • Why spread the bacteria out all over the agar plate? • Why put a disk with NO antibiotic on each agar plate? • Why put a disk with newly purchased, fresh antibiotic on each plate? • Why put so many disks on each plate? • Why wait 16-20 hours after you have put the bacteria and antibiotic disks on the plate?
Transcribed Image Text:Learning Task 16-03 - Antibiotic Effectiveness PART A • Record the steps that the laboratory technician follows in the video. • Highlight, by colour or bolding, the steps that ensure the experiment is sterile. Materials You will need: 6 millimeters filter paper disks, sterile Petri dishes, agar plates and no added antibiotics, sensitive and resistant bacterial cultures, an antibiotic to test and antibiotics control Steps 1. Pick colony and grow overnight 2. Dilute culture and grow to OD = 0.5 3. Prepare antibiotic stock 4. Add antibiotic to each disc 5. Dry discs at room temperature 6. Spread bacteria evenly on agar plate Place discs in center of sectioned areas 7. 8. Incubate plates 16-20h and measure zones PART B Re-watch the video and explain why the following steps are necessary: • Why use tweezers to put disks on the plate? • Why sterilize tweezers after each disk is put on the agar? • Why spread the bacteria out all over the agar plate? • Why put a disk with NO antibiotic on each agar plate? • Why put a disk with newly purchased, fresh antibiotic on each plate? • Why put so many disks on each plate? • Why wait 16-20 hours after you have put the bacteria and antibiotic disks on the plate?
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