PIGMENT SPOT Indoxyl Indigo White Indigo Yellow Indigotin

Principles Of Pharmacology Med Assist
6th Edition
ISBN:9781337512442
Author:RICE
Publisher:RICE
Chapter8: Forms Of Drugs And How They Act
Section: Chapter Questions
Problem 30RQ
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Heae
asiry
we aDe
2 bydro pbobic
silica gel
plates.
Solverd syslens
solvaol spplem easi
/one of
PAM) and other contaln-
ing
hyd nophobic silica plate can hold haydao phobr coponents
ard so hydrophobie com penuols
less, when wh@ oun tHhe solvent
only very
the compenadts
can move
and
which
move fca above
1 on the
A licatyel plate wll
be morie bydrophi'lic Cas we
cao cusing hydrophobic Silica plate)
Faom the plates o bseaved
ROSI Solvent stystems comporent
fasthest, means
we can
both PAMI and
See
In
D
bas
moved
57
more polaa , follo wed by
Components
C, B
and A.
obsaving the
Indigo yellow has move CA-OH
an ozy gen group capable gf doat29
Now
pigments presepl in indego
groupS
and
alsd
és Cnueleophilic),) hydsophilic gooups
hydrophilic , followad bay ndigo whete Cwith a -01
and 2-NH), then
more
Indoxy) C has
one -oH and
NH groups), and the last , least
indigot in Cunh a-NH)
-GH gooup IS
one
Po lag.
noore polaa than
NH gooup.
Transcribed Image Text:Heae asiry we aDe 2 bydro pbobic silica gel plates. Solverd syslens solvaol spplem easi /one of PAM) and other contaln- ing hyd nophobic silica plate can hold haydao phobr coponents ard so hydrophobie com penuols less, when wh@ oun tHhe solvent only very the compenadts can move and which move fca above 1 on the A licatyel plate wll be morie bydrophi'lic Cas we cao cusing hydrophobic Silica plate) Faom the plates o bseaved ROSI Solvent stystems comporent fasthest, means we can both PAMI and See In D bas moved 57 more polaa , follo wed by Components C, B and A. obsaving the Indigo yellow has move CA-OH an ozy gen group capable gf doat29 Now pigments presepl in indego groupS and alsd és Cnueleophilic),) hydsophilic gooups hydrophilic , followad bay ndigo whete Cwith a -01 and 2-NH), then more Indoxy) C has one -oH and NH groups), and the last , least indigot in Cunh a-NH) -GH gooup IS one Po lag. noore polaa than NH gooup.
This is Thin Layer Chromatography Indigo dye from Marsdenia tinctoria is used
2 hydrophobic silica gel plates are used. They then placed each plate in separate
developing chambers, 2 unknown solvent system. One containing solvent system
PAMI and the other containing solvent system ROSI.
SOLVENT
DISTANCE
SPOTS
SYSTEM
TRAVELLED, cm
solvent
6.4
A
1.4
PAMI
В
1.9
C
4.1
5.3
solvent
6.5
А
0.7
ROSI
В
1.7
В
2.5
В
A
3.0
A
PAMI
ROSI
The indigo mixture from the plant source is known to contain the following pigments.
OH
OH HO
Indoxyl
Indigo White
но
LOH
OH
Indigo Yellow
Indigotin
Based on the structures above, write the letter symbol of the spot corresponding to the
possible-identities of the pigments. Explain your answer.
PIGMENT
Indoxyl
Indigo White
Indigo Yellow
Indigotin
SPOT
Transcribed Image Text:This is Thin Layer Chromatography Indigo dye from Marsdenia tinctoria is used 2 hydrophobic silica gel plates are used. They then placed each plate in separate developing chambers, 2 unknown solvent system. One containing solvent system PAMI and the other containing solvent system ROSI. SOLVENT DISTANCE SPOTS SYSTEM TRAVELLED, cm solvent 6.4 A 1.4 PAMI В 1.9 C 4.1 5.3 solvent 6.5 А 0.7 ROSI В 1.7 В 2.5 В A 3.0 A PAMI ROSI The indigo mixture from the plant source is known to contain the following pigments. OH OH HO Indoxyl Indigo White но LOH OH Indigo Yellow Indigotin Based on the structures above, write the letter symbol of the spot corresponding to the possible-identities of the pigments. Explain your answer. PIGMENT Indoxyl Indigo White Indigo Yellow Indigotin SPOT
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