Plasmid A carries an ampicillin resistant gene (bla) and a gene for production of a GFP protein; this protein allows bacteria to fluoresce green when irradiated with UV light. If we transform a bacteria that does not have either of these genes or traits with plasmid A, the bacteria transformed with plasmid A should now (select both correct options). grow on plates containing kanamycin grow on plates containing ampicillin not fluoresce when irradiated with UV light fluoresce blue when irradiated with UV light fluoresce green when irradiated with UV light

Plasmid A carries an ampicillin resistant gene (bla) and a gene for production of a GFP protein; this protein allows bacteria to fluoresce green when irradiated with UV light. If we transform a bacteria that does not have either of these genes or traits with plasmid A, the bacteria transformed with plasmid A should now (select both correct options). grow on plates containing kanamycin grow on plates containing ampicillin not fluoresce when irradiated with UV light fluoresce blue when irradiated with UV light fluoresce green when irradiated with UV light

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Genes encoding toxins are often located on plasmids.A recent outbreak has just occurred in which a bacterium that is usually nonpathogenic is producing atoxin. Plasmid DNA can be isolated from this newlypathogenic bacterial strain and separated from thechromosomal DNA. To establish whether the plasmidDNA contains a gene encoding the toxin, you coulddetermine the sequence of the entire plasmid andsearch for a sequence that looks like other toxin genespreviously identified. An easier way exists to determine whether the plasmid DNA carries the gene(s)for the toxin; this strategy does not involve DNAsequence analysis. Describe this easier method.
To identify mutant genes, a wild-type bacterial genomiclibrary in plasmids is used to transform the mutant bacterialstrain. A transformed ________ in which the mutantphenotype is rescued to wild type likely harbors a plasmidcontaining a wild-type copy of the corresponding gene
A plasmid has two restriction cut sites for EcoRI and one restriction cut site for HindIII. The plasmid is subjected to PCR, and the region containing the three restriction cut sites is amplified, such that all three sites are present in the resulting amplicon. If the amplicon is incubated with EcoRI, how many fragments of DNA are expected to result from this digest experiment? a. 0 b. 1 c. 2 d. 3 e. 4
Find a plasmid map for pET11a and create a basic procedure for cloning a gene into this vector. Which selection method and substance would you use for this plasmid after transformation?
A researcher has a Trp− auxotrophic strain of E. coliwith a mutation in a single gene. To identify thatmutant gene, she uses a genomic library made from a wild-type version of that same strain to find plasmids that rescue the mutant phenotype. The result issurprising. She recovers 10 plasmids that provide aTrp+ phenotype, but six of the plasmids contain geneX, while the other four contain gene Y. Our scientisthas encountered a phenomenon called multicopysuppression, related to the fact that plasmids are usually present in several copies per bacterium. Becausethe genes in the plasmids are present in more thantheir usual single copy in the bacterial chromosome,more than the usual amount of Protein X or Protein Yis being produced from the plasmids. Sometimes,overexpression of one protein can rescue the mutantphenotype caused by loss of a different protein.Suggest at least two ways that our scientist could determine which of the two genes, gene X or gene Y, actually corresponds to the…
If you use the pUC18 vector to clone in the MCS region, predict the following: a) Do bacteria that are blue in color have a cloned insert? b)Do bacteria that are white in color have a cloned insert? c) If you were to grow these cells on Chloramphenicol (an antibiotic), would the bacteria with the pUC plasmid grow? Why or Why not?
A plasmid that is both ampicillin and tetracyclineresistant is cleaved with PstI, which cleaves within theampicillin resistance gene. The cut plasmid is ligated withPstI-digested Drosophila DNA to prepare a genomic library,and the mixture is used to transform E. coli K12. Question: How can you explain the presence of colonies thatare resistant to both antibiotics?
Construct a restriction map for Plasmid X to show the recognition sites for two commonly used restriction enzymes: EcoRI and BamHI. Plasmid X undigested Plasmid X digested with EcoRI Plasmid X digested with BamHI Plasmid X digested with EcoRI and BamHI 2200 bp 1000 bp 1200 bp 400 bp 1800 bp 200 bp 400 bp 600 bp 1000 bp
When a 5-kb circular plasmid is digested with a restriction enzyme that has three recognition sites on the plasmid, how many bands can be visualized on an agarose gel? a)1 b)2 c)5 d)3
Many studies have linked plasmids to bacterial resistance to antibiotics. Closured circular segments of double-stranded DNA, plasmids have several restriction enzyme cut sites along with genes that give the cell advantageous characteristics. Do you believe that fighting widespread antibiotic resistance may be accomplished by focusing on these specific DNA segments? In one or two paragraphs, formulate a well-supported opinion. Can you please provide a citation as well
This plasmid was digested using different restriction enzymes whose sites have been mapped. The plasmid is 7896 base pairs long. This is a long question so u can count this as two or even three but please answer the question? Determine the size (base pairs) and number of fragments that would be produced if the plasmid was digested with the following enzymes: a) EcoRI b) BamHI c) HindIII d) EcoRI and HindIII e)EcoRI, HindIII, and BamHI *Hint- this is actually an EASY question, since the restriction map is already drawn for you!
You are attempting to clone a 3 kb gene from the bacterial sp Microbacterium foliarum into the EcoRI site of the 6.0 kb plasmid shown. If you restrict the plasmid with EcoR1, how many bands will you obtain on agarose gel electrophoresis? Compare it with a plasmid in which no gene has been inserted. Draw a representative agarose gel showing the bands, and the direction of current flow as well point out the positive and negative electrodes