Question 3 Listen Imagine that you took a stock of 1,000,000 pfu/mL of bacteriophage and made a series of dilutions by taking 1mL from the stock and adding it to 9mL of fresh broth. You then mixed that tube and repeated 2 more times, each time taking 1mL and adding it to 9mL of fresh broth, for a total of 3 dilutions. THEN you added a few drops of E. coli and plated 1mL from your final tube on a TSA plate. What would you observe after 24 hours incubation? It might help to draw a picture. (How many plaques?) 1 10 100 1,000 10,000 100,000 1,000,000

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Imagine that you took a stock of 1,000,000 pfu/mL of bacteriophage and made a series of dilutions by taking 1mL
from the stock and adding it to 9mL of fresh broth. You then mixed that tube and repeated 2 more times, each time
taking 1mL and adding it to 9mL of fresh broth, for a total of 3 dilutions. THEN you added a few drops of E. coli and
plated 1mL from your final tube on a TSA plate. What would you observe after 24 hours incubation? It might help to
draw a picture. (How many plaques?)
1
10
100
1,000
10,000
100,000
1,000,000
Transcribed Image Text:Question 3 Listen Imagine that you took a stock of 1,000,000 pfu/mL of bacteriophage and made a series of dilutions by taking 1mL from the stock and adding it to 9mL of fresh broth. You then mixed that tube and repeated 2 more times, each time taking 1mL and adding it to 9mL of fresh broth, for a total of 3 dilutions. THEN you added a few drops of E. coli and plated 1mL from your final tube on a TSA plate. What would you observe after 24 hours incubation? It might help to draw a picture. (How many plaques?) 1 10 100 1,000 10,000 100,000 1,000,000
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