In this activity you will analyze the results of experiments that investigate nutritional requirement ofseveral mutant strains of yeast. The mutations in these strains cause a nutritional requirement for anamino acid, such that the strains will not grow in media that lack one specific amino acid. Any mutantthat has a nutritional requirement is called an auxotroph, and is incapable of growing in a "minimalmedium" containing only a carbon source (e.g., glucose), a simple nitrogen source (e.g., ammoniumsulfate), and various salts and minerals. Such strains can be supported on a medium supplementedwith only the missing nutrient or on a "rich" medium that contains amino acids, vitamins, nitrogenousbases, etc. (often in the form of an extract from yeast). The wild-type individual that can synthesizethe metabolic component is a prototroph, and is capable of growth on minimal medium.The mutant strains in this activity are unable to synthesize tryptophan, lysine, or histidine; oneprototrophic strain is included in the analysis.We start with a master plate that has 7 haploid strains of yeast (A-G), arranged in patches in thefollowing order (looking at the plate right side up with the upper surface of the agar facing you):A BCDEFGThe yeast strains from the master plate will be tested for their ability to grow on five different media.A very light inoculum of each strain will be transferred in the same pattern as above to the followingmedia: unsupplemented minimal medium, three minimal media each supplemented by one aminoacid (tryptophan, histidine, or lysine), and a rich medium. After inoculation, the plates are incubatedat 30°C for one or two days, and grow or absence of growth is recorded.Question 6: What do you think is the composition of the medium in the master plate (minimal,minimal supplemented with one amino acid, or rich)?RichO Minimal supplemented with one amino acidO Minimal

Nutrient media A nutrient media is a medium that is used to support microbial growth in…

Question 6: What do you think is the composition of the medium in the master plate (minimal, minimal supplemented with one amino acid, or rich)? Rich O Minimal supplemented with one amino acid Minimal

Question 6: What do you think is the composition of the medium in the master plate (minimal, minimal supplemented with one amino acid, or rich)? Rich O Minimal supplemented with one amino acid Minimal

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter15: From Dna To Protein

Section15.1: The Connection Between Dna, Rna, And Protein

Problem 1SB

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In this experiment, _____ plates were used to differentiate between red and white diploid yeast colonies. If a diploid colony grew on the MIN plate, this means the yeast ____synthesize _____
Which of the following would best ensure that a kidney cell and a liver cell from the same person would express different genes? Group of answer choices differences in introns unique sets of transcription factors they only have specific genes unique sets of promoters 2. Refer to the metabolic pathway illustrated. If A, B, and C are all required for growth, a strain mutant for the gene encoding enzyme A would be able to grow on medium supplemented with which of the following nutrient(s)?
When 1 million cells of a culture of haploid yeastcarrying a met− auxotrophic mutation were plated onpetri plates lacking methionine (Met), five coloniesgrew. You would expect cells in which the originalmet− mutation was reversed (by a base change back tothe original sequence) would grow on the media lackingmethionine, but some of these apparent reversions couldbe due to a mutation in a different gene that somehowsuppresses the original met− mutations. How wouldyou be able to determine if the mutations in your fivecolonies were due either to a precise reversion of theoriginal met− mutation or to the generation of a suppressor mutation in a gene on another chromosome?
Prototrophy is often the phenotype selected to detecttransformants. Prototrophic cells are used for donorDNA extraction; then this DNA is cloned and the clonesare added to an auxotrophic recipient culture. Successfultransformants are identified by plating the recipientculture on minimal medium and looking for colonies.What experimental design would you use to make surethat a colony that you hope is a transformant is not, infact,a. a prototrophic cell that has entered the recipientculture as a contaminant?b. a revertant (mutation back to prototrophy by a secondmutation in the originally mutated gene) of theauxotrophic mutation?
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Create workplan step by step on regenerating a BT maize plant selecting using phosphinothricin herbicide. Mention uses of agrobacterium , somatic embryogenesis, and construction of engineered TDNA for your workplan