Review innovative methods for growing microbes isolated from nature in the laboratory
Most microbes have not yet been cultured or grown under lab conditions. The great plate count anomaly is the discrepancy that is seen in between the number of microbial cells seen via a microscopic examination to that of the number of microbial colonies that can actually be cultivated from the same natural sample.
A microbe is said to be VBNC(viable but nonculturable) when they show motility when directly observed in their natural environment but is failed to be successfully cultivated in lab conditions. The gold standard method for the cultivation of microbes is the pure culture method. But there were also new and innovative methods being developed for culturing microbes.
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