Set up the spectrometer, finding an appropriate integration time, number of scans to average and record appropriate dark and reference spectra. Question 1: What effect does changing the integration time and number of scans averaged have on the noise in both the raw spectrum and absorbance spectrum? How might this effect the maximum and minimum sample concentrations that can be studied?
Set up the spectrometer, finding an appropriate integration time, number of scans to average and record appropriate dark and reference spectra. Question 1: What effect does changing the integration time and number of scans averaged have on the noise in both the raw spectrum and absorbance spectrum? How might this effect the maximum and minimum sample concentrations that can be studied?
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Transcribed Image Text:2. Set up the spectrometer, finding an appropriate integration time, number of
scans to average and record appropriate dark and reference spectra.
Question 1: What effect does changing the integration time and number of
scans averaged have on the noise in both the raw spectrum and absorbance
spectrum? How might this effect the maximum and minimum sample
concentrations that can be studied?
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