State the five basic steps of DNA fingerprinting using the RFLP method. Why do you think the PCR method is of more use in crime scene investigations?
State the five basic steps of DNA fingerprinting using the RFLP method. Why do you think the PCR method is of more use in crime scene investigations?
Introduction:
DNA is the genetic material that transfers from one to another present in the nucleus of the cell. It is comprised of nitrogenous bases (adenine, thymine, cytosine, and guanine), deoxyribose sugar, and a phosphate group.
Explanation:
DNA fingerprinting is a laboratory method used to build up a connection between natural proof and a suspect in a criminal examination.
RFLP was the primary technique utilized for DNA fingerprinting. This procedure designed in 1984 by the English researcher Alec Jeffreys during an investigation into genetic illnesses.
The means include:
- DNA extraction: first and foremost, DNA is separated from the blood, salivation, or different examples and sanitized.
- DNA fragmentation: The sanitized DNA is processed utilizing limited endonucleases.
- Gel electrophoresis: The limitation sections delivered during DNA fracture are examined utilizing gel electrophoresis.
- Visualization: The gel is treated with iridescent colors to make the DNA groups obvious.
- Examination
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