Table of BSA solution absorbance of concentration at 595nm. BSA Concentration (ug/ml) OD595nm Group 1 Group 2 Group 3 Grou 0.126 0.133 0.125 0.12 25 0.190 0.146 0.164 0.2- 50 0.298 0.209 0.185 0.25 75 0.291 0.224 0.242 0.3: 100 0.325 0.188 0.266 0.3- 150 0.222 0.181 0.268 0.3.
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- You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equationFive identical biood samples were analyzed to deterttmine cholesterol level in a patient The feilwing values were ohtained 157, 154, 155, 152, 155. Calculate the standand enor of the mean (SEM) fo thee dataTable 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…
- The strength of ionic interactions is weaker in water than inan anhydrous medium. Explain how water weakens theseinteractions.fill in the volumes required to make the various dilutions of Coomassie Blue and their respective concentrations. show sample calculations.if we need to prepare isotonic solution to the human blood plasma. How much Na (MW 58) we have to add to 0.5 L Distal water. And Why we use Na not K to prepare isotonic solution
- Calculate the pH of a buffer that contains 0.15 M MOPS anionic form and 0.25M MOPS zwitterion. The pKa is 7.2The glass electrode used in commercial pH meters gives an electrical response proportional to the concentration of hydrogen ion. To convert these responses to a pH reading, the electrode must be calibrated against standard solutions of known H+ concentration. Determine the weight in grams of sodium dihydrogen phosphate (NaH2PO4 · H2O; FW 138) and disodium hydrogen phosphate (Na2HPO4 ; FW 142) needed to prepare 1 L of a standard buffer at pH 7.00 with a total phosphate concentration of 0.100 M . See Problem 24 for the pKa values of phosphoric acid.Guanosine (C10H13N5O5) in solution has a maximum absorbance at a wavelength of 275 nm. The molar extinction co-efficient at this wavelength is 84M−1cm−1and the path length is 24.7 cm. Through the use of a spectrophotometer, it is found that the that A275= 1.48. What is the concentration of the guanosine solution in grams/litre? Molecular weights (g/mol): C-12, H-1, N-14, O-16 Select one: A. 0.201869 g/L B. 0.435188 g/L C. 0.059919 g/L D. 0.294046 g/L E. 0.000713 g/L
- An analysis done on total Mn2+ of an industrial effluent is found to be 8.5 mmol L-1. The effluent will be discharged in a lake of 15 mg L-1 fulvic acid (FA) content. What is the fraction of FA expected to bind to Manganese at pH-5. Assume that Mn2+ is the only cation present in Water Lake and that its concentration is 1/10th of that of the effluent after being diluted in the lake. Binding sites of FA = 4.5 mmol g -1 and K'f (MnFA) at pH 5 = 5.0 x 10^3 Select one: A. 0.51 B.51% C. None of the suggested answers D. 0.62 E.81%Put these aqueous solutions in order of decreasing freezing point 0.1 m NaCl 0.1 m MgCl2 0.1 m CH3OH 0.1 m Na3PO4I am unsure how to answer question number 2: What volume of 0.100 M HCl would have to be added to this buffering system, to reduce the pH to 7.1?