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than one answer is carrect. 1. An inducible enzyme is one that: A Is always under allosteric regulation. OC. Is synthesised only when it is needed. D. Is post-transcriptionally activated by a substrate. OF. None of the above. 2. Large amounts of B-galactosidase are synthesi sed by E. coli: OB. In the presence of lactose as sole carbon source. OC. Only when the medium also cantains peptone (a mixture of amino acide) as carbon Source. OD. In the presence of any metabolizable sugar, provided that peptone is present in the medium. OE. In a peptone medium also containing the lactose analogue isopropyl B-thiogalactoride, OF. In a glucose medi um also containing the lactose analogue isopropyl B-thiogalasteude 3. When E. coli is grown on a medium containing peptane (a rich mixture of amino acids) as the sole carbon source: OA. The lac repressor protein is synthesi sed constitutively. OB. The rate of synthesis ofthe lac repressor protein is increased OC. The lac repressor protein represses B-galactosidase activity by binding to the active site of the enzyme. 1 D. Peptone induces the synthesis of CAMP, which in turn induces the synthesis of the repressor protein. DE. The first product of the polycistronic lac structural gene is the repressor protein. OF. The operator is activated, leading to the synthesis of repres sor protein. 4. E. coli is able to synthesise B-galactosidase (in the absence of glucose) when OA. The regulator (la) gene binds the repressor protein O B. RNA polymerase bound to the promoter is able to move past the operator to the

than one answer is carrect. 1. An inducible enzyme is one that: A Is always under allosteric regulation. OC. Is synthesised only when it is needed. D. Is post-transcriptionally activated by a substrate. OF. None of the above. 2. Large amounts of B-galactosidase are synthesi sed by E. coli: OB. In the presence of lactose as sole carbon source. OC. Only when the medium also cantains peptone (a mixture of amino acide) as carbon Source. OD. In the presence of any metabolizable sugar, provided that peptone is present in the medium. OE. In a peptone medium also containing the lactose analogue isopropyl B-thiogalactoride, OF. In a glucose medi um also containing the lactose analogue isopropyl B-thiogalasteude 3. When E. coli is grown on a medium containing peptane (a rich mixture of amino acids) as the sole carbon source: OA. The lac repressor protein is synthesi sed constitutively. OB. The rate of synthesis ofthe lac repressor protein is increased OC. The lac repressor protein represses B-galactosidase activity by binding to the active site of the enzyme. 1 D. Peptone induces the synthesis of CAMP, which in turn induces the synthesis of the repressor protein. DE. The first product of the polycistronic lac structural gene is the repressor protein. OF. The operator is activated, leading to the synthesis of repres sor protein. 4. E. coli is able to synthesise B-galactosidase (in the absence of glucose) when OA. The regulator (la) gene binds the repressor protein O B. RNA polymerase bound to the promoter is able to move past the operator to the

Concepts of Biology
Concepts of Biology
1st Edition
ISBN:9781938168116
Author:Samantha Fowler, Rebecca Roush, James Wise
Publisher:Samantha Fowler, Rebecca Roush, James Wise
Chapter9: Molecular Biology
Section: Chapter Questions
Problem 10RQ: Control of gene expression in eukaryotic cells occurs at which level(s)? a. only the transcriptional...
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Put crosses against correct answers to the following questions. In some questions, more than one answer is carrect. 1. An inducible enzyme is one that: DA Is always under allosteric regulation. OC. Is synthesised only when it is needed. OD. Is post-transcriptionally activated by a substrate. OE. Is post-translati onally activated by a substrate. OF. None of the above. 2. Large amounts of B-galactosidase are syrthesised by E. coli: OA In the presence of glucose as sole carbon source. B. In the presence of lactose as sole carbon source. Oc. Only when the medium also cantains peptone (a mixture of amino acids) as carbon source. OD. In the presence of any metabolizable sugar, provided that peptone is present in the medium. OE. Ina peptone medium also containing the lactose analogue isopropyl B-thiogalactoside, OF. In a glucose medium also containing the lactose analogue isopropyl B-thiogalactoude 3. When E. coli is grown on a medium containing peptane (a rich mixture of amino acids) as the sole carbon source: OA The lac repressor protein is synthesised constitutively. OB. The rate of synthesis ofthe lac repressor protein is incre ased OC. The lac repressor protein represses B-galactosidase activity by binding to the active site of the enzyme. D. Peptone induces the synthesis of CAMP, which in turn induces the synthesis of the repressor protein. E. The first product of the polycistronic lac structural gene is the repressor protein. OF. The operator is activated, leading to the synthesis of repressor protein. 4. E. coli is able to synthesise B-galactosidase (in the absence of glucose) when OA. The regulator (la) gene binds the repressor protein OB. RNA polymerase bound to the promoter is able to move past the operator to the structural genes. OC. An inducer binds to the repressor protein. O D. The repressor protein is unable to bind to the operator. DE. The operator binds the inducer, hence preventing binding of the repressor protein. OF. The lac repressor protein binds to RNÅ polymerase, hence preventing synthesis of the MRNA far B-galactosidase. | 5. In the presence of an inducer, predict how the rate of B-galactosidase synthe sis in the following E. coli mutants would most likely differ from the wild type: OA Increase if the promoter of the lac operon is deleted. OB. Decrease if the promoter of the lac operon is deleted. OC. No change if the promoter of the lac operon is deleted. OD. Increase if the regulator (lasA gene is deleted. O E. Decrease if the regulator gene is deleted. O F. No change if the regulator gene is deleted.
Transcribed Image Text:Put crosses against correct answers to the following questions. In some questions, more than one answer is carrect. 1. An inducible enzyme is one that: DA Is always under allosteric regulation. OC. Is synthesised only when it is needed. OD. Is post-transcriptionally activated by a substrate. OE. Is post-translati onally activated by a substrate. OF. None of the above. 2. Large amounts of B-galactosidase are syrthesised by E. coli: OA In the presence of glucose as sole carbon source. B. In the presence of lactose as sole carbon source. Oc. Only when the medium also cantains peptone (a mixture of amino acids) as carbon source. OD. In the presence of any metabolizable sugar, provided that peptone is present in the medium. OE. Ina peptone medium also containing the lactose analogue isopropyl B-thiogalactoside, OF. In a glucose medium also containing the lactose analogue isopropyl B-thiogalactoude 3. When E. coli is grown on a medium containing peptane (a rich mixture of amino acids) as the sole carbon source: OA The lac repressor protein is synthesised constitutively. OB. The rate of synthesis ofthe lac repressor protein is incre ased OC. The lac repressor protein represses B-galactosidase activity by binding to the active site of the enzyme. D. Peptone induces the synthesis of CAMP, which in turn induces the synthesis of the repressor protein. E. The first product of the polycistronic lac structural gene is the repressor protein. OF. The operator is activated, leading to the synthesis of repressor protein. 4. E. coli is able to synthesise B-galactosidase (in the absence of glucose) when OA. The regulator (la) gene binds the repressor protein OB. RNA polymerase bound to the promoter is able to move past the operator to the structural genes. OC. An inducer binds to the repressor protein. O D. The repressor protein is unable to bind to the operator. DE. The operator binds the inducer, hence preventing binding of the repressor protein. OF. The lac repressor protein binds to RNÅ polymerase, hence preventing synthesis of the MRNA far B-galactosidase. | 5. In the presence of an inducer, predict how the rate of B-galactosidase synthe sis in the following E. coli mutants would most likely differ from the wild type: OA Increase if the promoter of the lac operon is deleted. OB. Decrease if the promoter of the lac operon is deleted. OC. No change if the promoter of the lac operon is deleted. OD. Increase if the regulator (lasA gene is deleted. O E. Decrease if the regulator gene is deleted. O F. No change if the regulator gene is deleted.
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