The 5’-ACTGCA DNA probe will hybridize with (stick to) which of the following DNA strands? a. 5’-TGACGT b. 3’-UGACGU c. 5’-GGCAAU d. 3’-TGACGT e. 5’-CCGUUA
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The 5’-ACTGCA DNA probe will hybridize with (stick to) which of the following DNA strands?
a. 5’-TGACGT
b. 3’-UGACGU
c. 5’-GGCAAU
d. 3’-TGACGT
e. 5’-CCGUUA
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Solved in 3 steps
- A DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA fragments? a. AGTCCGAAGTC c. GACTTCGGACT b. TCAGGCTTCAG d. UGAGGCUUGAGWhich one of the following statements is true? a. Dideoxynucleotides signal the end of DNA replication in a cell b. None of the provided answers are true c. The DNA sequence determined by an autoradiogram should be identical to the template strand DNA sequence d. The probe hybridization solution used in the Sothern blot technique is approximately pH 10.0 e. The transfer buffer used in the Southern blot technique is approximately pH 7.0You have just completed a plasmid DNA isolation procedure, but when you measured the amount DNA you isolated using the NanoSpec, it had an A260/A280 ratio of 0.83 which indicates that it includes a lot of protein in addition to the plasmid DNA. This tells you something went wrong in your isolation - maybe you missed a step, or a solution was made incorrectly, etc. What is one possible specific error that could have been made during your DNA isolation procedure? Explain why you think this.
- n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genesTranscribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’DNA Polymerase holoenzymes used for DNA replication recognizes A. double-stranded sequences as starting points B. methylated lipids as start points C. acetylated lipids as start points D. single stranded sequences as starting points
- Which of the following would you need to clone a human gene? Choose all that apply a) A surrogate mother b) A plasmid vector (or other type of DNA vector, e.g. phage, BAC) c) A donor goat nucleus d) A restriction enzyme e) Human DNA f) An enucleated egg g) DNA ligaseThe diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction? Group of answer choices a. The number of copies triples (or triplicates). b. The number of copies does not change. c. The number of copies quadruples (or quadruplicates). d. The number of copies doubles (or duplicates). e. The number of copies halves.Which of the following would you need to clone a goat? Choose all that apply a) A restriction enzyme b) A surrogate mother c) A plasmid vector (or other type of DNA vector, e.g. phage, BAC) d) A donor goat nucleus e) Human DNA f) DNA ligase g) An enucleated egg
- Arrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,bWhat are the sticky ends of the restriction fragments? Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases? a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d. The DNA band detected by Southern blot is different from that by Northern blot
