Answered: The leaf explants cultured on a culture…

The leaf explants cultured on a culture medium turned black and caused the medium turned to brown colour after 5 days of culture. Explain this phenomenon and suggest two ways to overcome this problem

Basic Clinical Lab Competencies for Respiratory Care: An Integrated Approach

5th Edition

ISBN:9781285244662

Author:White

Publisher:White

Chapter15: Humidity And Aerosol Therapy

Section: Chapter Questions

Problem 4SEPT

See similar textbooks

Related questions

Q: Explain growth-cycle phases for a batch cultivation. Does Penicillin production occur in log or…

A: Germs need certain conditions in order to grow, and these conditions are not unique to all bacteria.…

Q: Explain the reason behind the positive result in Benedict's test in leaves and roots of a plant.

A: Introduction:- Benedict's test is a chemical test that can be used to determine whether or not an…

Q: Which drug would cause disruption to an amoeba's movement? (Note: these drugs are real and their…

A: The pseudopodia is formed by the actions of actin polymerization forming the microfilaments. The…

Q: The supernatant from the first centrifugation of the barely seeds extract will most probably:

A: Barley seed extract contains good amount of starch and starch digesting alpha and beta amylase but…

Q: What initiatives could be taken if plant tissue cultures are suffering severely due to alkaloid…

A: Cell culture is the technique in which cells isolated from multicellular organisms are grown in…

Q: To test the technique's efficacy, leaf and stem explants were sterilised using a surface…

A: Sterilization is the process of removal of contaminants.

Q: Imagine you pull away the cover slip and drip 10 drops of deionized water on the young Elodea leaf,…

A: Plasmolysis can be reversed if the cell is placed in a hypotonic solution. Stomata help keep water…

Q: One of the most resistant biological material present in the exine of pollen grain is: (a)…

A: Pollen grains are present inside the anther. They consist of male gametes. The pollen grains are of…

Q: Macro and micro elements are most important in plant's nutrition. Explain which form these elements…

A: MS medium (Murashige and Skoog Medium) is the most used plant tissue culture medium that provides…

Q: explain the strategies halophiles have evolved to cope with osmotic stress and why these strategies…

A: Halophiles comes from the Greek word 'salt loving'. These are extremophiles who live in high salt…

Q: Briefly discuss one extraction method used in studying/analyzing plant phytochemicals.

A: Plants are a reservoir of biochemical that can be used by humans in pharmaceutical and agrochemical…

Q: CALLUS AND CELL SUSPENSION IN SELECTED PLANTS OBJECTIVES : To prepare plant materials as a source…

A: Callus is mass of undifferentiated cells. For the formation of callus cells which are totipotent are…

Q: (Plant Pathology) What is the importance of culture media in the diagnosis of plant disease?

A: It is critical that we understand microbes and their applications since they may be both useful and…

Q: What plant process involved in your experiment aside from transpiration?

A: Plants are vital living forms that belong to the Plantae taxonomic kingdom and are further classed…

Q: Amy carried out an experiment to study the effect of two types of media: (a) MS basal medium and (b)…

A: Plant tissue culture is a artificial laboratory culture of any part or tissue of the plant or the…

Q: Plant Pathology: Is Koch’s Postulate a relevant technique in diagnosing plant disease caused by…

A: Plant pathology is a branch of botany and primarily deals with the scientific study of diseases in…

Q: Briefly explain why research on the molecular mechanism of stomatalclosure might be of future use in…

A: Introduction: Leaves in plants are responsible to perform photosynthesis and gas exchange.…

Q: discuss the implications of having a good culture medium for the diagnosis of plant diseases?

A: Plant diseases are caused by different reasons ranging from pest infestations till waterlogged roots…

Q: When Using I2KI test why should pigments be extracted from the leaf before adding I2KI

A: Question asked: When Using I2KI test why should pigments be extracted from the leaf before adding…

Q: After performing surface sterilisation on leaves, the leaf explants were cultured on a medium…

Q: 1) Consider the two titles below, both relating to the same experiment: • "Environmental controls of…

A: Stoma (stomata) is a small pore found in the epidermis of leaves and other organs and plays a…

Q: Plant varieties may be distinguished, one from another, byobserving properties of their leaves.a.…

A: All the substances of Earth possess some special properties by which they can be identified. Based…

Q: Which of the following is considered as an appropriate plant extraction technique? 1.…

A: The basic steps of extraction are:- penetration of solvent into a solid matrix dissolution of…

Q: laborate the advantage and disadvantages of Nano-Plant Growth Regulator

A: Plant growth regulators are the substances that support the growth of plants and are being applied…

Q: (f) Given alongside is an experimental set up to study a particular process: Water drops Bell jar…

A: (I) The act of perspiring or we call it Transpiration. (ii) 'Transpiration is the loss of water as…

Q: o get a salt-tolerant plant, in which stage we have to induce salt in the plant tissue culture…

A: Plant tissue culture is a technique in biotechnology which enables to produce plants, seeds etc in…

Q: scientific literature

A: Logical Inquiry One thing is normal to all types of science: an extreme objective to know. Interest…

Q: GRAPH 1. AMOUNT OF CHLOROPHYLL WITH DIFFERENT AMOUNTS OF LIGHT 600 - Low light --- Moderate light…

A: The given curve has amount of chlorophyll (on y-axis) and time for which light is exposed on to…

Q: Dark roast coffee-wet method Start with the part of interest growing on the plant. Take me…

A: The dark roast processing method is a process in which the fermentation of the coffee are done by…

Q: Explant can be aa) Cut part of the plant used in tissue cultureb) Plant extract used in tissue…

A: In biology, explant is defined as a piece or pieces of tissue or organ removed from a plant or…

Q: What are the methods in measuring total leaf carbon content and lignin content from a leaf and…

A: Carbon content is the total amount of carbon present in the leaf and the lignin content is the total…

Q: What would be main consideration in the media composition forgrowth-linked products vs products…

A: The synthesis of growth-linked products (which include primary metabolites) is directly correlated…

Q: You are interested in the effect of both soil copper concentration and pH on the growth of plants…

A: The given setup is considered as one of the simplest growth setup. The growth rate is measured. The…

Q: is a physicochemical modification of soil or synthesis from the precursor materials. O Addition O…

A: SOIL - soils are the products of complex interactions between the lithosphere , atmosphere and…

Q: Why is leguminous crop rotation used in agriculture?

A: Leguminous plants or crops are those that have swelled-like structures in their root known as…

Q: Identify the stages of mitotic division observed in one of SimpLENI's slides labeled as A, B, and C.…

A: The stage labeled as A is Telophase. Telophase is the 4th stage of mitosis where the sister…

Q: Some friends are trying to make wine in their basement. They've added yeast to a sweet grape juice…

A: Yeast is widely used as a microbial starter in fermentation processes. Wineries and other…

Q: n the experiment, it showed that the rate of decomposition in plant material declines as the process…

A: Decomposition : It is the process where by the dead tissues break down and are converted into…

Q: When plant cells are in a hypotonic medium, they (a) undergo plasmolysis (b) build up turgor…

A: Hypotonic solution: The concentration of solutes in a hypotonic solution is lower as compared to in…

Q: major obstacles to the use of tissue culture in crop improvement. Explain why this process is…

A: In Vivo means the plants which are grown in the normal conditions where is ex vivo who means when…

Q: Define the process of transpiration and state the possible advantages and disadvantages of…

A: This question is about transpiration.

Q: Explain why micropropagation of banana is a better way of producing planting materials than…

A: Micropropagation is a method of tissue culture where a small part of the parent plant is used to…

Q: 1a)A surface sterilisation protocol was carried out to sterilise leaf and stem explants for…

A: Surface sterilization is the process of removal of contaminants present on the surface of leaves and…

Q: Describe the effects of the following on photosythesis in the intact leaf a)leaf properties…

A: Introduction: It is the process by which plants use energy from sunlight to produce sugar and on…

Q: Two groups of tomatoes were grown under laboratoryconditions, one with humus added to the soil and…

A: The plants are multicellular eukaryotes that belong to the Plantae kingdom. The plants are…

Q: plant suspension culture was used to study the effect of three types of media on the growth of the…

A: A plant cell suspension culture is a sterile system normally initiated by aseptically placing…

Q: if a student conducts an experiment cultivating a cell. at the initial time the number of cells was…

A: Bacteria: Bacteria are single-celled organisms that are extremely small.Bacteria are present almost…

Q: Which plant tissue culture technique is best for Heritiera fomes (Sundari plant in Sundarban)…

A: Plant tissue culture or micropropagation is a technique that utilizes the totipotency of plants and…

Concept explainers

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.

Question

The leaf explants cultured on a culture medium turned black and caused the medium turned to brown colour after 5 days of culture. Explain this phenomenon and suggest two ways to overcome this problem

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

What initiatives could be taken if plant tissue cultures are suffering severely due to alkaloid production and accumulation in the medium.
A study was conducted on the impact of phytohormones on medicinal stem explants.The stem explants were cultured onto MS media containingdifferent types of phytohormones as listed in Table below. Predict the resultsobtained after 4 weeks with justifications and conclude the results youpredicted
Explain why preparation of stock solutions for macronutrients, micronutrients and vitamins required in preparing a plant tissue culture medium is recommended
Give me three examples on the cultivation of any tropical plants in Asia by using micropropagation methods.
To test the technique's efficacy, leaf and stem explants were sterilised using a surface sterilisation protocol. Before being treated with 50 percent Clorox for 20 minutes, all explants were properly cleansed with distilled water. After that, the explants were washed with distilled water before being cultivated on Murashige and Skoog (MS) basal medium plates.For each type of explant, a total of five plates were created, each with ten explants. Table shows the findings obtained after a week. Discuss and draw conclusions from the findings.
Which plant tissue culture technique is best for Heritiera fomes (Sundari plant in Sundarban) regeneration?
Discuss, in details, the characteristics and function of the unique feature which enables the identification of the eucaryotic cell type(s) observed with the onion and/or Elodea cells in the water preparation. (i.e., the observable feature(s) that identified the specimen as a plant cell).
Explain why micropropagation of banana is a better way of producing planting materials than traditional methodologies. Describe how to carry out micropropagation for this particular crop, including the type of explant to be used and the use of phytohormones or additives in the culture medium needed for each culture stage, along with justifications.
Amy carried out an experiment to study the effect of two types of media: (a) MS basal medium and (b) MS basal medium + X on the germinated seedlings. After 4 weeks, the seedlings cultured on (a) were found to be significant shorter than those that were cultured on (b). Identify X with justification.
SimpLENI will be competing in a Lugaw Cooking Competition in May. She will be using onions as a special ingredient and garnish in her recipe. She then planted onions in two soil treatments labelled as ANGAT and UNITY. After a week, SimpLENI noticed that her onions in ANGAT increased in number compared to that of onions in UNITY. She then hypothesized that the soil treatments affected the cellular division in the roots of onions contributing to the difference in yield. To prove this, SimpLENI prepared longitudinal sections of onion root tips. Support SimpLENI in her experiment and win the competition by answering the following questions:
Solar-induced fluorescence has a linear relationship with a plant's gross primary production. Explain why the relationship is linear?
5a)Micropropagation is a beneficial approach in crop propagation as it couldincrease the yield of production besides obtaining disease-free plantingmaterial. Elaborate and discuss this statement.