Answered: The results of Figure 2 highlights the…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

The results of Figure 2 highlights the antibacterial activity of cacay oil. Since the method involves adding cacay oil to a "broth" medium, it can be inferred that cacay oil is hydrophilic. Agree or Not?

Transcribed Image Text:Antibacterial activity in the sample with higher values of polyunsaturated fatty acids was per- formed according to the methodology described by the Clinical and Laboratory Standards Institute (CLSI) [23], with modifications. Six potentially pathogenic bacterial strains were tested: three Gram-negative (Escherichia coli ATCC 25912, Pseudomonas aeruginosa ATCC 27853, and Salmonella paratyphi ATCC 14028) and three Gram-positive (Bacillus cereus ATCC 11778, Enterococcus faecalis ATCC 29212, and Staphylococcus aureus ATCC 6538). The microorganism suspension in Mueller Hinton (MH) broth (Merck, Darmstadt, Ger- many) was added to different sample concentrations (2.25–144.00 mg/mL) dissolved in 1% (v/ v) tween 80 in a 96-well microplate. The microplates were then incubated at 35°C under shak- ing at 200 rpm (Quimis, Q816M20, Diadema, Brazil). The optical density at 595 nm was deter- mined at times 0 and 24 h. Wells containing MH broth, microorganisms, and saline solution 0.9% with 1% tween 80 were considered as positive growth control (100% bacterial growth). Vancomycin (0.4 mg/ ml) was added to the Gram-positive and gentamicin (0.3 mg/mL) to the Gram-negative bacte- ria for the microbial sensibility profile control. The inhibition percentage of bacterial growth was calculated according to Eq 1: [AAbs- AAbsample x 100 AAbs Inhibition of bacterial growth (%) = (1) Where AAbspc represents the absorbance variation in the positive control (100% bacterial growth), and Aabs,ample the absorbance variation in the sample.