There are several proteins that exist in nature that are fluorescent, which means they absorb visible light or other electromagnetic radiation and reemit that light, usually at a longer wavelength. One of the more prevalent fluorescent proteins is GFP, or green fluorescent protein, which emits bright green fluorescent light when exposed to light in the blue to ultraviolet region of the electromagnetic spectrum (see the photo) The main absorption, or excitation, wavelength for one type of GFP is 395 nm. (a) What is the energy (in eV) of a photon in this absorbed light? The emission spectrum of the light from the GFP peaks near 509 nm, which is in the shorter wavelength (higher energy) portion of the green part of the visible spectrum. (b) What is the energy (in eV) of a photon in the emitted light? (c) If the emitted light corresponded to the longest wavelength in the Balmer series from a hydrogen-like atom, what would be the effective value of Z for this atom? (Note: Z will not be a whole number in this case.) The mice on the left and right have been genetically modified to express GFP when illuminated with UV light. Their exposed skin cells (eyes, nose, ears, and tail) glow bright green due to fluorescence and indicate healthy cells. The mice also contain subcutaneous breast cancer tumor cells that glow red (not shown), so they can be easily distinguished from healthy cells. This aids researchers in tracking the metastization process of the cancer. The mouse in the middle has not been modified. (a) Number (b) Number i i Ingrid Moen, Charlotte Jevne, Jian Wang, Karl-Henning Kalland, Martha Chekenya, Lars A Akslen, Linda Sleire, Per ØEnger, Rolf K Reed, Anne M Øyan and Linda EB Stuhr: Gene expression in tumor cells and stroma in dsRed 4T1 tumors in eGFP-expressing mice with and without enhanced oxygenation. https://commons. wikimedia.org/wiki/File:GFP_Mice_01.jpg (c) Number i Units Units Units
There are several proteins that exist in nature that are fluorescent, which means they absorb visible light or other electromagnetic radiation and reemit that light, usually at a longer wavelength. One of the more prevalent fluorescent proteins is GFP, or green fluorescent protein, which emits bright green fluorescent light when exposed to light in the blue to ultraviolet region of the electromagnetic spectrum (see the photo) The main absorption, or excitation, wavelength for one type of GFP is 395 nm. (a) What is the energy (in eV) of a photon in this absorbed light? The emission spectrum of the light from the GFP peaks near 509 nm, which is in the shorter wavelength (higher energy) portion of the green part of the visible spectrum. (b) What is the energy (in eV) of a photon in the emitted light? (c) If the emitted light corresponded to the longest wavelength in the Balmer series from a hydrogen-like atom, what would be the effective value of Z for this atom? (Note: Z will not be a whole number in this case.) The mice on the left and right have been genetically modified to express GFP when illuminated with UV light. Their exposed skin cells (eyes, nose, ears, and tail) glow bright green due to fluorescence and indicate healthy cells. The mice also contain subcutaneous breast cancer tumor cells that glow red (not shown), so they can be easily distinguished from healthy cells. This aids researchers in tracking the metastization process of the cancer. The mouse in the middle has not been modified. (a) Number (b) Number i i Ingrid Moen, Charlotte Jevne, Jian Wang, Karl-Henning Kalland, Martha Chekenya, Lars A Akslen, Linda Sleire, Per ØEnger, Rolf K Reed, Anne M Øyan and Linda EB Stuhr: Gene expression in tumor cells and stroma in dsRed 4T1 tumors in eGFP-expressing mice with and without enhanced oxygenation. https://commons. wikimedia.org/wiki/File:GFP_Mice_01.jpg (c) Number i Units Units Units
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Transcribed Image Text:There are several proteins that exist in nature that are fluorescent, which means they absorb visible light or
other electromagnetic radiation and reemit that light, usually at a longer wavelength. One of the more
prevalent fluorescent proteins is GFP, or green fluorescent protein, which emits bright green fluorescent
light when exposed to light in the blue to ultraviolet region of the electromagnetic spectrum (see the photo).
The main absorption, or excitation, wavelength for one type of GFP is 395 nm. (a) What is the energy (in eV)
of a photon in this absorbed light? The emission spectrum of the light from the GFP peaks near 509 nm,
which is in the shorter wavelength (higher energy) portion of the green part of the visible spectrum. (b)
What is the energy (in eV) of a photon in the emitted light? (c) If the emitted light corresponded to the
longest wavelength in the Balmer series from a hydrogen-like atom, what would be the effective value of Z
for this atom? (Note: Z will not be a whole number in this case.)
The mice on the left and right have been genetically modified to express GFP when illuminated with UV
light. Their exposed skin cells (eyes, nose, ears, and tail) glow bright green due to fluorescence and indicate
healthy cells. The mice also contain subcutaneous breast cancer tumor cells that glow red (not shown), so
they can be easily distinguished from healthy cells. This aids researchers in tracking the metastization
process of the cancer. The mouse in the middle has not been modified.
(a) Number
i
(b) Number i
(c) Number
Ingrid Moen, Charlotte Jevne, Jian Wang, Karl-Henning Kalland,
Martha Chekenya, Lars A Akslen, Linda Sleire, Per ØEnger, Rolf
K Reed, Anne M Øyan and Linda EB Stuhr: Gene expression in
tumor cells and stroma in dsRed 4T1 tumors in eGFP-expressing
mice with and without enhanced oxygenation. https://commons.
wikimedia.org/wiki/File:GFP_Mice_01.jpg
i
Units
Units
Units
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