What are the two type of viral vector involved in gene therapy trials?

Q: What is a simple way of making a knockout mouse in terms of DNA recombinant technology?

A: A knockout mouse, or knock-out mouse, is a genetically modified mouse in which researchers have…

Q: What is the definition of antibiotic resistance gene?

A: Antibiotics are the antimicrobial chemicals that can be bactericidal (kill the bacteria) or…

Q: What is one stumbling block in AAV gene therapy in some patients?

A: Biotechnology is technology that utilizes biological systems, living organisms or parts of this to…

Q: What are the steps in making an mRNA vaccine?

A: mRNA vaccines combine ideal immunological houses with an exceptional protection profile and the…

Q: What are the types ofantigenic agents that mayconstitute vaccines?

A: An antigen is defined as any substance, either pathogen, cell metabolite, pollutant, pollen grains,…

Q: Which of the following viruses have been used as vectors for gene delivery?

A: Gene delivery is a process of introducing the foreign genetic materials which are DNA and RNA into…

Q: Mention any three vector-less methods that are used to introduce recombinant DNA into a competent…

A: Micropropagation ,electroporation and gene gun methods are three vectorless methods that are used to…

Q: what are the Resistant strain emergence to DNA inhibitors

A: Drug resistance is the reduction in effectiveness of a medication such as antimicrobial in treating…

Q: How do 3D protein modeling tools help in the development of drugs or vaccine?

A: The 3D structures of the desired target proteins, drugs and epitopes can be predicted and modeled…

Q: What does the enzyme reverse transcriptase do and why is it agood target for anti-HIV drugs?

A: A virus is a submicroscopic infectious agent. It replicates only inside the living cells of an…

Q: Explain how viral vectors can be used for gene therapy, and provide two examples of commonly used…

A: The two methods of gene transfer used in gene therapy are: The viral-mediated gene transfer…

Q: hat are some of the challenges of using recombinant molecules to treatment of HIV?

A: HIV (human immunodeficiency virus) affects immune cells that aid in the body's ability to fight…

Q: Will genome editing emerge as the safest and most reliablemethod of gene therapy, rendering other…

A: Genome editing is an advanced molecular technique where the DNA sequences are altered through the…

Q: How do 3D protein modeling tools help in the development of drugs or vaccines?

A: Drug discovery and vaccine development requires a detailed understanding of the cellular…

Q: what are the ethical and cultural behind genetic modification CRISPR Cas9?

A: CRISPR Cas 9 is a family of DNA sequences in bacteria. These are the part of the bacterial immune…

Q: plain the fundamental difference between CRISPR/Cas9 and in vivo adenovirus-based gene therapy

A: All biological data of AN organism is held on within the biomolecule known as DNA. gene is the…

Q: Describe how a plaque assay is used to detect and estimate the numbers of phage particles.

A: Plaque is a term used in microbiology for a clear zone on a bacterial culture plate that indicates…

Q: How are inactivated vaccine and mRNA vaccine produced? From what you know about DNA technology and…

A: Inactivated vaccines are made up of virus particles, bacteria, or other pathogens that have been…

Q: What is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

A: Gene therapy is a method that allows the correction of the defective gene. In gene therapy, it is…

Q: List examples of how in vivo and ex vivo gene therapy has been used to treat human disease.

A: In vivo gene therapy refers to treatment that is provided to the patient directly. The targeted…

Q: What is a microsatellite?  Draw what a microsatellite looks like in a zone of inhibition in a disk…

A: Microsatellite- It is repetitive DNA in which motifs of DNA are repeated.

Q: Give three important characteristics of cloning vectors.

A: Cloning is an important process in which bacterial cells containing recombinant DNA can be readily…

Q: Give three reasons why Salmonella strains are very useful for mutagenesis studies

A: Ans- The standard set of bacterial strains that are used in the mutagenicity testing of…

Q: What changes can be made to an mRNA transcript to be used in a vaccine in order to change its…

A: mRNA is basically composed of protein encoding ORF, surrounded by 5′ and 3′ UTRs, and a 7-methyl…

Q: How applications of CRISPR-Cas technology is used ?

A: A group of DNA sequences known as CRISPR can be discovered in the genomic of prokaryotic organisms…

Q: Define how DNA can be inserted into vectors ?

A:

Q: Describe the molecular features of a BAC cloning vector. What is theprimary advantage of a BAC…

A: Cloning is defined as the process of formation of the individuals possessing identical DNA. This…

Q: What types of viruses are used for gene therapy, and how are they manipulated to make them useful?

A: Gene therapy is the technique used to cure or prevent the disease by introducing the normal gene in…

Q: What are the implications of resistance gene transfer for the future practice of medicine?

A: Bacteria multiply quickly and have a variety of methods for exchanging DNA. This is how they are…

Q: What is the purpose of including a tube in the phage assay in which only the E. coli culture is…

A: Insects that feed on fecal, dung material and raw sewage are termed as coprophagous insects. These…

Q: During the first successful gene therapy trial in which Ashanti DeSilva was treated for SCID, did…

A: Gene therapy is the process by which defective or non-functional gene can be replaced by a normal…

Q: Which PCR variants are used for the accurate diagnosis of virus and how virus quantification is…

A: Introduction: PCR or polymerase chain reaction is a laboratory method used to amplify the genetic…

Q: What

A: Vaccines help to protect the human body by preparing it to fight foreign pathogens or viruses in…

Q: How does the molecular mechanism of the CRISPR-Cas systemuse a viral DNA sequence against that same…

A: A virus is a small microscopic microorganism that can replicate inside living organism that is host.…

Q: using the concept of gene therapy,describe how to design a vehicle to target SARS CoV-2 RNA?

A: Gene therapy is used a gene delivery system to insert a gene into the nucleus of the cell for the…

Q: Using the concept of gene therapy describe how you can design a vehicle to target SARS-CoV-2 RNA?

A: Gene therapy is a treatment for the disease, require a gene delivery system for target cells to…

Q: Outline a PCR-based approach to screen for colonies transformed with recombinant expression plasmid…

A: In order to select for colonies which are recombinant and contain the plasmid DNA can be separated…

Q: In the plaque assay, exactly what makes up a single plaque?

A: Viruses are non-cellular pathogenic particles. Viruses cannot survive on their own as they lack cell…

Q: How do you think can genetic engineering help in making vaccines for diseases such as COVID-19?

A: A "vaccine" is a chemical that is utilized in the body to produce antidotes and offers protection…

Q: What is the genetic material of a virus? How does that material act in viralreproduction?

A: A virus is an acellular particle and an obligate parasite.

Q: what are the advantages and disadvantages of CRISPR cas9 technology?

A: CRISPR -Clustered Regularly Interspersed Short Palindromic Repeats The CRISPR cas9 is a technology…

Q: Differentiate between plasmids, phage-based cloning vectors, cosmids, and artificial chromosomes in…

A: Plasmids Structure -  are small, circular, double stranded, non-chromosomal DNA molecules which…

Q: How do you design a reporter assay for Serpina1?

A: The gene has two parts. The first is the coding sequence which gets transcribed to RNA and…

Q: How can gene therapy help in the cure of COVID-19?

A: Gene therapy is a therapeutic strategy that involves the introduction of gene fragments to…

Q: Explain Gene therapy with viral vectors?

A: Gene therapy is a medical technique that is used in the treatment of diseases by the help of genes.…

Q: Explain the RNA-seq methods ?

A: RNA-seq is a powerful tool for transcriptome analysis that can provide insights into gene expression…

Q: Viral gene delivery vectors are favored for therapeutic applications because of their efficiency and…

A: Viral gene delivery vectors or the viral vectors are the most preferred method of gene transduction…

Q: In the gene therapy trials for SCIDS, what DNA was added to the patients’ cells? Could this same…

A: "Severe combined immunodeficiency" (SCID) is an X-linked disorder of the immune system that occurs…

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