what does it mean if a bacteria tested negative for oxidase

Aquaculture Science
3rd Edition
ISBN:9781133558347
Author:Parker
Publisher:Parker
Chapter9: Fundamentals Of Nutrition In Aquaculture
Section: Chapter Questions
Problem 10E
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what does it mean if a bacteria tested negative for oxidase 

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Step 1

The oxidase test in bacteria is performed to confirm the production of cytochrome c oxidase. It is a complex transmembrane protein and the end enzyme in the electron transport process of bacteria. The purpose of this test is:

  • Identification of anaerobes by testing the production of cytochrome c oxidase.
  • The differentiation between the Pseudomonas and other bacterial colonies.
Step 2

The experiment gives us two observations positive or negative. When a bacteria gives positive results in the oxidase test it means the use of oxygen as an end molecule in the electron transport process. When a bacteria gives negative results there could be a chance of aerobic, anaerobic, or facultative bacteria in the sample. It confirms that these bacterias do not use cytochrome c oxidase for the electron transport process.

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