Chapter9: Fundamentals Of Nutrition In Aquaculture
Section: Chapter Questions
Problem 10E
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Question
what does it mean if a bacteria tested negative for oxidase
Expert Solution
Step 1
The oxidase test in bacteria is performed to confirm the production of cytochrome c oxidase. It is a complex transmembrane protein and the end enzyme in the electron transport process of bacteria. The purpose of this test is:
- Identification of anaerobes by testing the production of cytochrome c oxidase.
- The differentiation between the Pseudomonas and other bacterial colonies.
Step 2
The experiment gives us two observations positive or negative. When a bacteria gives positive results in the oxidase test it means the use of oxygen as an end molecule in the electron transport process. When a bacteria gives negative results there could be a chance of aerobic, anaerobic, or facultative bacteria in the sample. It confirms that these bacterias do not use cytochrome c oxidase for the electron transport process.
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