What is blue/white screening? What is the key feature of a plasmid that is used for it?

Q: What conditions are making bacteria competent to taking up a foreign plasmid? Please answer asap

A: Natural genetic competence is the ability of cells to bind and taken up exogenous DNA. It is…

Q: How is Ti plasmid of Agrobacterium tumefaciens modified to convert it into a cloning vector?

A: Agrobacterium tumefaciens is a well-studied species of the gram-negative genus of bacteria, known as…

Q: What are the features of a plasmid being used as a cloning vector?

A: Some Features of a plasmid which have been used as a cloning vector are: Selectable markers…

Q: Define the following terms: Plasmid Restriction Enzyme Standard Curve

A: Recombinant DNA technology is similar to gene cloning that makes use of restriction enzymes to cut…

Q: How is the copy number of plasmid vector and yield of the recombinant protein related to each other?

A: In biotechnology, plasmid refers to the double stranded, circular DNA of bacteria; when these…

Q: which is the delivery techniques can bbe used to best transfer recombinant plasmids into E.coli…

A: ANSWER) The process of transferring the recombinant plasmids into the E.coli cells is called…

Q: What role does plasmids play in genetic engineering?

A: A plasmid is a small, circular, double-stranded extra chromosomal DNA molecule present within the…

Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: What is Ti plasmid ?

A: The biochemical molecule that is built up with “two polynucleotide chains” is called…

Q: What does the F plasmid code for?

A: The plasmid is defined as a small, circular, extra-chromosomal, double-stranded DNA molecule present…

Q: Plasmids are the only vectors currently available for use in recombinant procedures.True or false?

A: The technology used for joining different DNA molecules from different species is known as…

Q: How is a gene inserted into a plasmid cloning vector?

A: Apart from cell's chromosomal DNA, a double-stranded circular DNA molecule is present in yeast,…

Q: state two ways one can extract an insert from a plasmid

A: a plasmid is typically a tiny extrachromosomal DNA molecule that is found within the cell and is…

Q: Why are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the…

A: Cell lysis is frequently the very first step in most purification techniques for nucleic acids,…

Q: What is one way to determine whether a bacterial culture has receiveda recombinant plasmid?

A: Plasmids are small, extra chromosomal, and double stranded DNA structures that can replicate…

Q: How does restriction enzyme digestion allow for the determination of the correct plasmid construct…

A: Restriction enzymes are proteins that cut DNA at specific sequences. These enzymes are found in…

Q: Why is DNA microarray an important tool and explain How is DNA microarray used in detecting cancer?

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: Briefly describe two different methods for inserting foreign DNA into plasmids, giving the strengths…

A: The plasmid is typically a small circular deoxyribonucleic acid (DNA) strand in the cytoplasm of the…

Q: Why are plasmids used as vector for DNA Recombination? What other vectors can  be used?

A: Plasmids are the extrachromosomal DNA that can replicate independently. They contain an origin of…

Q: If the cloning was successful and the plasmid contained the elements needed for blue-white…

A: The screening of transformants after the cloning is a critical step as it allows the identification…

Q: What is restriction fragment length polymorphism and which method do we use to detect it?

A: Restrictions length polymorphism (RFLP) is a kind of polymorphism that outcomes from variety in the…

Q: .Describe the method you would use to test for the uptake of a plasmid with a DNA insert

A: Introduction: The introduction of recombinant DNA into a suitable host cell is followed by the…

Q: Where is the F plasmid located in an Hfr cell? Where is the F plasmid located in an F+ cell?

A: Prokaryotic cells are unicellular organisms that differ from multicellular organisms in composition…

Q: which parts would a plasmid vector with 2500 bp have in E. coli

A: A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's…

Q: In the alkaline plasmid screen, what two things separate plasmid from chromosomal DNAs?

A: Following is the procedure for the separation of plasmid by the alkaline lysis method.

Q: Explain the THREE (3) important regions of this plasmid that enable it to work efficiently as a…

A: Plasmid is a self replicating generally circular DNA molecule that can be used to carry a foreign…

Q: What are the essential elements of a plasmid cloning vector? Discuss the importance of each element…

A: Essential elements of plasmid cloning vector  Presence of Origin of replication for autonomous…

Q: What characteristics of plasmids makes them good cloning vectors? Discuss.

A: Genetic engineering is the branch of biology which deals with the process of making manipulation in…

Q: What is the Ti plasmid and how has it been of use ingenetic engineering?

A: A genetically engineered organism is the result of recombinant DNA technology that involves the…

Q: Figure 1 above is showing the plasmid map of pUC18. Discuss how screening is carried out if this…

A: pUC18/19 is an artificial plasmid cloning vector. This plasmid vector comprises of Ampicillin [AmpR}…

Q: If a F plasmid does not have the gene encoding TraJ, this F plasmid would

A: Conjugation is a major mechanism for dissemination of antibiotic resistance and virulence among…

Q: Why choose vectors and plasmids as a method of module introduction/expression?

A: Introduction  Plasmid Vectors: these are like the artificial vehicle to deliver our gene of insert…

Q: What are the functions of Glacial acetic acid and potassium acetate on plasmid DNA analysis

A: Plasmid isolation uses many salts and detergent for gradual separation of DNA from proteins and cell…

Q: What is a plasmid?

A: Prokaryotes are the less advanced organism as compared to the eukaryotes. They don't have well…

Q: In the plaque assay, what is the precise origin of a single plaque?

A: Answer: Introduction: Virus is an intracellular infectious parasite consist of only one type of…

Q: PLease explain What characteristics of plasmids makes them good cloning vectors? Discuss

A: Plasmids are small extrachromosomal DNA molecules that can reproduce independently of their…

Q: Besides plasmid, what other vectors could you use to amplify your gene of interest? Name THREE (3)…

A: A vector is any vehicle, often a virus or a plasmid that is used to ferry a desired DNA sequence…

Q: How is a specific probe obtained?

A: Probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a…

Q: What is SDS and what part does it play in the alkaline lysis method of preparing plasmid DNA from a…

A: DNA (deoxyribonucleic acid) was discovered by Friedrich Miescher. Nucleotides are the structural…

Q: Why are antibiotic resistance genes usually included in plasmids used for genetic transformation?

A: Bacterial transformation is the process of transferring the genetic material from one bacterium to…

Q: What is the purpose of ampicillin in the pGLO lab, where is the ampicillin locates, how it can be…

A:

Q: What is a plasmid? What are the minimum 3 components that a recombinant plasmid used for genetic…

A: Biotechnology is innovation that uses natural frameworks, living beings or parts of this to create…

Q: What is probe? Why it is used in the library screening ?

A: Biotechnology is the practice of using living organisms such as animals, plants, microbes, and…

Q: What are some potential difficulties in using plasmid vectors and bacterial host cells to produce…

A: Recombinant DNA technology involves integrating DNA molecules from two different species and…

Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.