What is this organism? And what other test could be done to confirm it's identification? 1.Gram stain - positive cocci, chains Catalase - weak positive Hemolysis - beta BE - positive NaCl- positive Bacitracin - no zone of inhibition PYR - positive Answer: Enterococcus spp. CAMP test to verify the identity 2.Gram positive cocci, chains Catalase - negative Hemolysis - alpha BE - negative NaCl - negative P disk - resistant Bile Solubility - negative Answer - viridans group, Use PYR test to verify
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What is this organism? And what other test could be done to confirm it's identification?
1.
Catalase - weak positive
Hemolysis - beta
BE - positive
NaCl- positive
Bacitracin - no zone of inhibition
PYR - positive
Answer: Enterococcus spp. CAMP test to verify the identity
2.Gram positive cocci, chains
Catalase - negative
Hemolysis - alpha
BE - negative
NaCl - negative
P disk - resistant
Bile Solubility - negative
Answer - viridans group, Use PYR test to verify
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- discuss the clinical significance, principles, reagents (if any) and positive and negative results of the following laboratory tests for Gram positive cocci organisms: Bile esculin test 5% salt turbidity or tolerance test PYRase test Pyruvate broth testIdentification of Gram-positive cocci: You have isolated a catalase-negative bacterium that doesn't grow well in 6.5% NaCl medium. Which test would you expect to give you a presumptive identification? (i.e. The first tests tell you the genus, which test would identify a species of that genus) CAMP Test Coagulase Tube Test PYR Test Novobiocin Sensitivity TestThis is a catalase-positive, coagulase-negative, gram-positive coccus isolated from a urine specimen from a 20-year-old female college student. The image shown is a Mueller Hinton plate streaked with a 0.5 MacFarland standardized inoculum and a 5 microgram disk of novobiocin after overnight incubation. What is the identification of the isolate? Please select the single best answer Staphylococcus epidermidis Staphylococcus aureus Staphylococcus saprophyticus Staphylococcus lugdenensis
- Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition. Is the test tube a A) broth, B) slant, or C) deep agar medium? Why is a “needle” used to inoculate?1. Illustrate in a diagram the identification scheme from gram-positive cocci.2. Discuss the principle involved in the biochemical tests in identifying species of staphylococci.3. Illustrate in a diagram the identification scheme for Streptococci.4. Discuss the principle involved in the biochemical tests in identifying species of Streptococci.An outbreak of Methicillin-resistant Staphylococcus aureus (MRSA) in the surgical ward at the hospital forces the hospital administrator to cancel and postpone all non-essential surgeries until the source of infection has been located and for measure put into place. Bacterial samples obtained from swab of wad staff equipment and surfaces arr brought to the lab for analysis. 1. What testing method will you employ to determine the level of exposure? 2 How would a positive test present? 3. How would a negative test present? 4. What recommendations would you make in your report?
- EXPLAIN THE PURPOSE OF THE METHYL RED AND VOGES-PROSKAUER TESTS IN DETERMINING THE IDENTITY OF GRAM NEGATIVE ORGANISMS IN THE FAMILY ENTEROBACTERIACEAE. INCLUDE IN YOUR ANSWER WHY METHYL RED IS USED RATHER THAN PHENOL RED. WHAT ORGANISM IN THE FAMILY IS USED AS A CONTROL WHEN RUNNING THE METHYL TEST. IF THE METHYL RED TEST IS NEGATIVE, EXPLAIN WHY THE VOGES-PROSKAUER TEST IS RUN AND INCLUDE WHAT COLOR CHANGE INDICATES A POSITIVE TEST. ACCOUNT FOR THE COLOR DIFFERENCES IN THE NEGATIVE VOGES-PROSKAURER TESTS. WHAT REAGENTS (COMMON NAME) WERE USED IN THE VOGES -PROSKAUER TEST.I tested organism growth on the MSA plate for strep. pyongenes, strep. pneumonia, s. aureus, and straph. epidermidis and my results were that streptococci organism did not grow or change color and the s.aureus changed color (yellow) and s.epi there was no change in color but they both showed growth. 1. If an unknown gram-positive bacterium was given, what would it mean if it grew on MSA? Name the organisms used above My answer: that it would be either s.aureus or s. epidermis because they are selective for salt-tolerant bacteria and can grow on mannitol 2. what would it mean if it both grew on MSA and turned yellow? Can you add to my answer or correct it. Thank you!List the following to determine the unknown bacteria: Streptococcus mutans 1) Morphology & Characteristics 2) the different biological tests to be done
- Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme Test Unknown Isolate B 1 Luminescence Positive 2 Cell Shape Straight Rods 3 Gram Staining Negative 4 Oxygen Requirement Facultative Anaerobe 5 Motility (Hanging Drop) Motile 6 Growth at 0% NaCl Negative 7 3% NaCl Positive 8 6 % NaCl Positive 9 Accumulation of PHB Positive 10 Flagella 1 Polar 11 Oxidase Positive 12 Nitrate Reduction 13 Gelatinase Negative 14 Growth at 35OC Positive 15 Growth at 4OC Negative 16 Methyl Red Positive 17 Voges-Proskauer Positive 18 Catalase Positive 19 Starch Hydrolysis Negative 20 Lysine…At autopsy, the most prominent findings were bronchopneumonia with focal organization and hemorrhage in the right lung. Stains of the lung tissue were negative by Gram, methenamine silver, and acid-fast methods, but Dieterle silver stains revealed short bacilli. Lung cultures yielded Gram-negative bacilli, which grew aerobically on buffered charcoal-yeast extract, but not on blood or chocolate agar. The organisms resembled Legionella, but failed to stain with immunofluorescence conjugates for Legionella pneumophila and multiple other species (L minded, L longbeachae, L gormanii, L dumoffi, L bozemanii). The organism was sent to the Centers for Disease Control and Prevention, where it was eventually identified as a new species of Legionella. What is the most probable source of this man's infection? Family member Water Food Insect Bioterrorism Dark-field microscopy may be used to diagnose spirochetes in which of the following scenarios? To detect spirochetes in the blood of a…A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?