What strategy does a genetically encoded calcium indicator look like to allow fluorescence imaging of only one cell type in an acute slice of the brain? A.The use of fluorescent protein expression inhibitors in other cells B.The injection of a recombinant virus causing the death of other cells C.The use of a promoter specific to these cells D.Activation of membrane receptors specific to these cells

What strategy does a genetically encoded calcium indicator look like to allow fluorescence imaging of only one cell type in an acute slice of the brain? A.The use of fluorescent protein expression inhibitors in other cells B.The injection of a recombinant virus causing the death of other cells C.The use of a promoter specific to these cells D.Activation of membrane receptors specific to these cells

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter9: Molecular Biology

Section: Chapter Questions

Problem 6RQ: A promoter is ______. a. a specific sequence of DNA nucleotides b. a specific sequence of RNA...

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A promoter is ______. a. a specific sequence of DNA nucleotides b. a specific sequence of RNA nucleotides c. a protein that binds to DNA d. an enzyme that synthesizes RNA
You are curious to identify the region of the gene X sequence that serves as an enhancer for gene expression. Design an experiment to investigate this issue.
You are studying a new gene that is expressed within the adipose tissue of humans during the development of diabetes. Specifically, you want to learn why gene expression levels are very high in those people who develop diabetes. What DNA sequence would you concentrate on? Group of answer choices A specific response elements B the TATA box C enhancer sequences D promoter proximal elements E metal response elements
A mutation occurs in a gene, resulting in it being expressed at the wrong time during development. Where is the mutation most likely to be found? a) In the transcription start site (TSS) b) In a TF binding site in the cis-regulatory region c) In the coding sequence d) In the 5' UTR
What is the advantage of using the neo gene to disrupt the function of a gene in knockout mice? a. The neo gene produces an antibiotic that kills unwanted cells. b. The neo gene is the right size for disabling other genes. c. The neo gene provides a selectable marker for finding cells that contain the disabled gene. d. The neo gene produces a toxin that inhibits transcription of the target gene
Yamanaka’s 2012 Nobel Prize was awarded for hisa. successful cloning of Dolly the sheep.b. demonstration that transcription factors canepigenetically reprogram adult cells.c. invention of somatic cell nuclear transfer technology.d. use of cloning to cure cystic fibrosis
a) What is a gene promoter? b) What proteins bind to a promoter? c) What do those proteins do to control gene expression?
Which of the following statements about the DNA in one of your brain cells is true? (A) Most of the DNA codes for protein. (B) The majority of genes are likely to be transcribed. (C) It is the same as the DNA in one of your liver cells. (D) Each gene lies immediately adjacent to an enhancer.
Suggest a direct experiment to prove that p53 binding at gene promoters affects the level of gene expression of the corresponding genes.
Why is regulating transcription the main way that cells control gene expression? A. Because transcription is the last step in gene expression, stopping here ensures that the cell has a stockpile of proteins to prepare them from all unexpected environmental changes. B. Because transcription involves interactions with DNA, preventing transcription reduces the changes of mutation in the cell’s genome. C. Because transcription is the first step in gene expression, stopping at transcription reduces the amount of energy and resources used by producing unnecessary gene products. D. Because transcription is the shortest step in gene expression, preventing transcription has little effect on the rate of protein production.
Which of the following are possible reasons that a cell would regulate its expression of a gene? (Select all that apply.) (a) an increased need for a particular enzyme (b) a decreased need for a particular enzyme (c) increasing temperature in the external environment (d) changing needs as an organism ages (e) death