when various strains of lambda phage are seeded on a lawn of e.coli, they can form clear or turbid plaques. Explain the difference between the two types of plaques. can all bacteriophage form clear and turbid plaques?
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
when various strains of lambda phage are seeded on a lawn of e.coli, they can form clear or turbid plaques.
Explain the difference between the two types of plaques. can all bacteriophage form clear and turbid plaques?
Bacteriophages are the viruses that infect and replicate within the bacterial host. They are of two types, virulent phages that undergoes lytic cycle (lysis of host) and temperate phage that undergo lysogenic cycle (phage remains dormant inside the host)
Lambda phage or coliphage λ is a type of bacteriophage that infects Escherichia coli. The Lambda phage is divided into protein heads (capsid) that encloses double stranded DNA, tail and the tail fibres. This virus is a temperate phage therefore, upon infecting the host bacteria and taking over the mechanism it resides within the genome as pro phage and does not cause lysis of the host. This stage is called lysogeny.
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